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1: Gene. 1991 Oct 30;107(1):161-4.Links

Molecular cloning of the Candida maltosa ADE1 gene.

Sasnauskas K, Jomantiene R, Geneviciute E, Januska A, Lebedys J.

Department of Molecular Biology, Institute of Applied Enzymology, Vilnius, Lithuanian Republic.

The structural gene (ADE1) encoding phosphoribosyl-aminoimidazole-succinocarboxamide synthetase (SAICAR synthetase; EC 6.3.2.6) in Candida maltosa has been isolated by functional complementation of an ade1 strain of Saccharomyces cerevisiae. The gene was localized on a 2.5-kb BamHI DNA fragment. Nucleotide sequence analysis of the cloned gene has revealed an open reading frame encoding a protein (SAICAR synthetase) with an Mr of 32,751. The codon bias index, 0.68, indicates that the ADE1 gene is a moderately highly expressed gene. The cloned gene shows 63.5% nt identity and 65.2% deduced amino acid identity with the S. cerevisiae ADE1 gene which encodes the same enzymatic activity. The gene may be used as a convenient genetic marker for construction of a new host-vector system for C. maltosa.

PMID: 1743515 [PubMed - indexed for MEDLINE]