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Molecular cloning and characterization of a Ca2+/calmodulin-insensitive adenylyl cyclase from rat brain.
Howard Hughes Medical School, Baltimore, MD.
Biochemical, immunological, and molecular cloning studies have suggested the existence of multiple forms of adenylyl cyclase (EC 4.6.1.1). An adenylyl cyclase cDNA clone (type II) was isolated from a rat brain library and found to encode a protein of 1090 amino acids that was homologous to but distinct from the previously described Ca2+/calmodulin-stimulated adenylyl cyclase from bovine brain. Expression of the type II cDNA in an insect cell line resulted in an increased level of adenylyl cyclase activity that was insensitive to Ca2+/calmodulin. Addition of activated Gs alpha protein to type II-containing membranes increased enzyme activity. The mRNA encoding the type II protein was expressed at high levels in brain tissue and at low levels in olfactory epithelium and lung. The existence of multiple adenylyl cyclase enzymes may provide for complex and distinct modes of biochemical regulation of cAMP levels in the brain.
PMID: 1719547 [PubMed - indexed for MEDLINE]
PMCID: PMC52890
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Cited by 19 PubMed Central articles
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Pharmacological characterization of adenosine receptors in PGT-beta mouse pineal gland tumour cells.
Suh BC, Kim TD, Lee JU, Seong JK, Kim KT.
Br J Pharmacol. 2001 Sep; 134(1):132-42.
[Br J Pharmacol. 2001]
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Catalytic mechanism of the adenylyl and guanylyl cyclases: modeling and mutational analysis.
Liu Y, Ruoho AE, Rao VD, Hurley JH.
Proc Natl Acad Sci U S A. 1997 Dec 9; 94(25):13414-9.
[Proc Natl Acad Sci U S A. 1997]
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Characterization of soluble forms of nonchimeric type V adenylyl cyclases.
Scholich K, Barbier AJ, Mullenix JB, Patel TB.
Proc Natl Acad Sci U S A. 1997 Apr 1; 94(7):2915-20.
[Proc Natl Acad Sci U S A. 1997]
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