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Molecular cloning and sequence analysis of cDNA encoding delta 4-3-ketosteroid 5 beta-reductase of rat liver.
Department of Oral and Maxillofacial Surgery II, Hiroshima University School of Dentistry, Japan.
A cDNA clone encoding delta 4-3-ketosteroid 5 beta-reductase was isolated from rat liver cDNA libraries using antibodies specific for the enzyme and oligonucleotides as probes. The cDNA contained 981-base pair open reading frame encoding 327 amino acid residues (Mr 37,376) and an unusually long 3'-untranslated region rich in AT sequence in the total length of 3189 base pairs. The predicted amino acid sequence contains the sequences similar to the putative NADPH- and steroid-binding regions.
PMID: 1710579 [PubMed - indexed for MEDLINE]
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Cited by 5 PubMed Central articles
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Crystal structure of human liver Delta4-3-ketosteroid 5beta-reductase (AKR1D1) and implications for substrate binding and catalysis.
Di Costanzo L, Drury JE, Penning TM, Christianson DW.
J Biol Chem. 2008 Jun 13; 283(24):16830-9. Epub 2008 Apr 11.
[J Biol Chem. 2008]
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Purification from rat liver of a novel constitutively expressed member of the aldo-keto reductase 7 family that is widely distributed in extrahepatic tissues.
Kelly VP, Ireland LS, Ellis EM, Hayes JD.
Biochem J. 2000 Jun 1; 348 Pt 2:389-400.
[Biochem J. 2000]
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ReviewComparative anatomy of the aldo-keto reductase superfamily.
Jez JM, Bennett MJ, Schlegel BP, Lewis M, Penning TM.
Biochem J. 1997 Sep 15; 326 ( Pt 3):625-36.
[Biochem J. 1997]
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