Sequence and tissue-specific expression of a putat...[Plant Mol Biol. 1991]

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1: Plant Mol Biol. 1991 Jan;16(1):171-4.Links

Sequence and tissue-specific expression of a putative peroxidase gene from wheat (Triticum aestivum L.).

Hertig C, Rebmann G, Bull J, Mauch F, Dudler R.

Institute for Plant Biology, University of Zürich, Switzerland.

We have used a cDNA clone encoding a pathogen-induced putative wheat peroxidase to screen a genomic library of wheat (Triticum aestivum L. cv. Cheyenne) and isolated one positive clone, lambda POX1. Sequence analysis revealed that this clone contains a gene encoding a putative peroxidase with a calculated pI of 8.1 which exhibits 58% and 83% sequence identity to the amino acid sequence of the turnip (Brassica rapa) peroxidase and a pathogen-induced putative wheat peroxidase, respectively. The two introns in the wheat gene are at the same positions as introns in the peroxidase genes of tomato and horseradish. Results of S1-mapping experiments suggest that this gene is neither pathogen- nor wound-induced in leaves but is constitutively expressed in roots.

PMID: 1653627 [PubMed - indexed for MEDLINE]

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Sequence and tissue-specific expression of a putative peroxidase gene from wheat (Triticum aestivum L.).

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