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1: Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15093-8. Epub 2005 Oct 7.Click here to read Click here to read Links
Erratum in:
Proc Natl Acad Sci U S A. 2005 Dec 6;102(49):17882.

p600, a unique protein required for membrane morphogenesis and cell survival.

Nakatani Y, Konishi H, Vassilev A, Kurooka H, Ishiguro K, Sawada J, Ikura T, Korsmeyer SJ, Qin J, Herlitz AM.

Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA. nakatani@mac.com

In this article, we identify and characterize p600, a unique 600-kDa retinoblastoma protein- and calmodulin-binding protein. In the nucleus, p600 and retinoblastoma protein seem to act as a chromatin scaffold. In the cytoplasm, p600 and clathrin form a meshwork structure, which could contribute to cytoskeletal organization and membrane morphogenesis. Reduced expression of p600 with interference RNA abrogates integrin-mediated ruffled membrane formation and, furthermore, prevents activation of integrin-mediated survival pathways. Consequently, knockdown of p600 sensitizes cells to apoptosis induced by cell detachment. These findings provide mechanistic insight into the regulation of membrane-proximal events in tumorigenesis.

PMID: 16214886 [PubMed - indexed for MEDLINE]

PMCID: PMC1247991