ADAM22 plays an important role in cell adhesion and spreading with the assistance of 14-3-3.
Zhu P,
Sang Y,
Xu H,
Zhao J,
Xu R,
Sun Y,
Xu T,
Wang X,
Chen L,
Feng H,
Li C,
Zhao S.
The State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China. pzhu@cbr.med.harvard.edu
Cellular adhesion plays important roles in a variety of biological processes. The ADAM family contains disintegrin-like and metalloproteinase-like domains which potentially have cell adhesion and protease activities. Recent studies suggest that the interaction between 14-3-3zeta and ADAM22cyt can regulate cell adhesion and spreading, therefore it has a potential role in neural development and function. 14-3-3 family has seven highly conserved members that regulate various cellular functions. Using yeast two-hybrid method, we identified that ADAM22cyt bound some other 14-3-3 family members. The interaction was further confirmed by in vitro protein pull-down assay and co-immunoprecipitation. We also found that the overexpression of exogenous ADAM22 in HEK293 cells could significantly enhance cell adhesion and spreading, compared with the truncated ADAM22 lack of 14-3-3 binding motifs. These results strongly demonstrated a functional role for ADAM22/14-3-3 in cell adhesion and spreading.
PMID: 15882968 [PubMed - indexed for MEDLINE]