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1: FEBS Lett. 2004 Oct 8;576(1-2):251-5.Click here to read Links

Laccase from Melanocarpus albomyces binds effectively to cellulose.

Kiiskinen LL, Palonen H, Linder M, Viikari L, Kruus K.

VTT Biotechnology, P.O. Box 1500, Fin-02044 VTT, Finland.

A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one-site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non-specific protein or 0.1-0.5 M Na2SO4. No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases. Copyright 2004 Federation of European Biochemical Societies

PMID: 15474046 [PubMed - indexed for MEDLINE]