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Structure and expression of the barley lipid transfer protein gene Ltp1.
Carlsberg Research Laboratory, Copenhagen, Denmark.
We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of Ltp1 showed that it contains an open reading frame of 351 bp interrupted by a single intron of 133 bp. Transient expression assays indicated that 702 bp of the 5' upstream region of Ltp1 is sufficient to direct aleurone-specific expression during late seed development and early germination.
PMID: 1536930 [PubMed - indexed for MEDLINE]
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Cited by 2 PubMed Central articles
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Tissue-specific expression and promoter analysis of the tobacco Itp1 gene.
Canevascini S, Caderas D, Mandel T, Fleming AJ, Dupuis I, Kuhlemeier C.
Plant Physiol. 1996 Oct; 112(2):513-24.
[Plant Physiol. 1996]
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Solution structure and lipid binding of a nonspecific lipid transfer protein extracted from maize seeds.
Gomar J, Petit MC, Sodano P, Sy D, Marion D, Kader JC, Vovelle F, Ptak M.
Protein Sci. 1996 Apr; 5(4):565-77.
[Protein Sci. 1996]