Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding.
Amit I,
Yakir L,
Katz M,
Zwang Y,
Marmor MD,
Citri A,
Shtiegman K,
Alroy I,
Tuvia S,
Reiss Y,
Roubini E,
Cohen M,
Wides R,
Bacharach E,
Schubert U,
Yarden Y.
Department of Biological Regulation, The Weizmann Institute of Science, Rehovot 76100, Israel.
The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane. Copyright 2004 Cold Spring Harbor Laboratory Press ISSN
PMID: 15256501 [PubMed - indexed for MEDLINE]
PMCID: PMC478194