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1: Biochem Biophys Res Commun. 2004 Jul 30;320(3):760-5.Click here to read Links

Daxx-mediated transcriptional repression of MMP1 gene is reversed by SPOP.

Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701, Republic of Korea.

Daxx-mediated transcriptional repression was modulated by a speckled POZ domain protein SPOP which was first identified as an autoantigen from the serum of a scleroderma patient. This is the first report on the biochemical and functional interactions between Daxx and SPOP. The COOH-terminal region of Daxx interacts with the NH2-terminal region of SPOP. SPOP reversed the transcriptional repression mediated by Daxx which binds with ETS1 transcription factor to repress ETS1-responsive gene expression. Mutagenesis study suggests that the ability of SPOP to self-associate as well as its ability to bind with Daxx was important for the modulation of Daxx-mediated transcriptional repression.

PMID: 15240113 [PubMed - indexed for MEDLINE]