Voltage-gated sodium channel expressed in cultured human smooth muscle cells: involvement of SCN9A.
Jo T,
Nagata T,
Iida H,
Imuta H,
Iwasawa K,
Ma J,
Hara K,
Omata M,
Nagai R,
Takizawa H,
Nagase T,
Nakajima T.
Department of Cardiovascular, Respiratory Medicine and Gastroenterology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Voltage-gated Na(+) channel (I(Na)) is expressed under culture conditions in human smooth muscle cells (hSMCs) such as coronary myocytes. The aim of this study is to clarify the physiological, pharmacological and molecular characteristics of I(Na) expressed in cultured hSMCs obtained from bronchus, main pulmonary and coronary artery. I(Na), was recorded in these hSMCs and inhibited by tetrodotoxin (TTX) with an IC(50) value of approximately 10 nM. Reverse transcriptase/polymerase chain reaction (RT-PCR) analysis of mRNA showed the prominent expression of transcripts for SCN9A, which was consistent with the results of real-time quantitative RT-PCR. These results provide novel evidence that TTX-sensitive Na(+) channel expressed in cultured hSMCs is mainly composed of Na(v)1.7.
PMID: 15178348 [PubMed - indexed for MEDLINE]