Exploratory and confirmatory gene expression profiling of mac1Delta.
De Freitas JM,
Kim JH,
Poynton H,
Su T,
Wintz H,
Fox T,
Holman P,
Loguinov A,
Keles S,
van der Laan M,
Vulpe C.
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA.
Exploratory outlier identification methods and confirmatory gene expression studies showed induction of the iron regulon in Saccharomyces cerevisiae lacking Mac1p, a copper-responsive transcription factor. The Aft1p/Aft2p binding motif was the most discriminating motif between up- and down-regulated genes, and we identified new genes potentially regulated by Aft1p/Aft2p. In addition, multiple genes encoding proteins containing Fe-S clusters were down-regulated suggesting metabolic reorganization to conserve iron in mac1Delta. Null mutants of each of the differentially expressed genes were characterized for copper- or iron-related phenotypes. New or additional support for a role in copper and iron homeostasis is provided in this study for the gene products of AKR1, MRS4, PCA1, SSU1, TIS11, YBR047W, YHL035C, YHR045W, YLR047C, YLR126C, and YTP1.
PMID: 14534306 [PubMed - indexed for MEDLINE]