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Sequence of the gene encoding type F neurotoxin of Clostridium botulinum.
Department of Microbiology, AFRC Institute of Food Research, Reading, UK.
Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin determined. Analysis of the nucleotide sequence demonstrated the presence of an open frame encoding a protein of 1274 amino acids, similar to other botulinum neurotoxins. Upstream of the toxin gene is the end of an open reading frame which encodes the C-terminus of a protein with homology to non-toxic-non-hemagglutinin component of type C progenitor toxin.
PMID: 1398040 [PubMed - indexed for MEDLINE]
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Cited by 6 PubMed Central articles
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Sequence variation within botulinum neurotoxin serotypes impacts antibody binding and neutralization.
Smith TJ, Lou J, Geren IN, Forsyth CM, Tsai R, Laporte SL, Tepp WH, Bradshaw M, Johnson EA, Smith LA, et al.
Infect Immun. 2005 Sep; 73(9):5450-7.
[Infect Immun. 2005]
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Simple and efficient method for heterologous expression of clostridial proteins.
Zdanovsky AG, Zdanovskaia MV.
Appl Environ Microbiol. 2000 Aug; 66(8):3166-73.
[Appl Environ Microbiol. 2000]
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Molecular cloning of the gene encoding the mosaic neurotoxin, composed of parts of botulinum neurotoxin types C1 and D, and PCR detection of this gene from Clostridium botulinum type C organisms.
Moriishi K, Koura M, Fujii N, Fujinaga Y, Inoue K, Syuto B, Oguma K.
Appl Environ Microbiol. 1996 Feb; 62(2):662-7.
[Appl Environ Microbiol. 1996]
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