Cloning and sequencing of glutamate mutase compone...[FEBS Lett. 1992]

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1: FEBS Lett. 1992 Sep 28;310(2):167-70. Links

Cloning and sequencing of glutamate mutase component S from Clostridium tetanomorphum. Homologies with other cobalamin-dependent enzymes.

Marsh EN, Holloway DE.

Department of Biochemistry, University of Cambridge, UK.

The gene encoding component S, the small subunit, of glutamate mutase, an adenosylcobalamin (coenzyme B12)-dependent enzyme from Clostridium tetanomorphum has been cloned and its nucleotide sequence determined. The mutS gene encodes a protein of 137 amino acid residues, with M(r) 14,748. The deduced amino acid sequence showed homology with the C-terminal portion of adenosylcobalamin-dependent methylmalonyl-CoA mutase [1989, Biochem. J. 260, 345-352] and a region of cobalamin-dependent methionine synthase which has been shown to bind cobalamin [1989, J. Biol. Chem 264, 13888-13895].

PMID: 1397267 [PubMed - indexed for MEDLINE]

Cloning, sequencing and expression in Escherichia coli of the gene encoding component S of the coenzyme B12-dependent glutamate mutase from Clostridium cochlearium.
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[FEMS Microbiol Lett. 1994]
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[FEBS Lett. 1993]
Adenosylcobalamin-dependent glutamate mutase: properties of a fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit.
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[Biochem J. 1996]
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[Curr Opin Struct Biol. 1994]
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Cloning and sequencing of glutamate mutase component S from Clostridium tetanomorphum. Homologies with other cobalamin-dependent enzymes.

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Cited by 9 PubMed Central articles

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