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1: Chin J Biotechnol. 1992;8(4):235-45.Links

Cloning, mapping, and sequencing of 3' and its flanking region of bovine alpha-s1 casein gene.

Chen R, Wang B, Zhang Y, Liu W, Zhang J, Lao W.

Institute of Biophysics, CAS, Beijing, China.

A positive clone was isolated from a bovine genomic library in EMBL3 with the probe of alpha-s1 casein gene cDNA. The results from both restriction endonuclease digestion and Southern hybridization probed with the upstream and downstream fragments from the termination codon of bovine alpha-s1 casein gene cDNA suggested that the clone contained 3.5 kb downstream and 10.2 kb upstream fragments from the termination codon of alpha-s1 casein gene. Some divergences in restriction endonucleases sites, a few deletions, and many point mutations were found in introns and exons when compared with those which were published.

PMID: 1343827 [PubMed - indexed for MEDLINE]