-
Cloning and analysis of a Candida maltosa gene which confers resistance to formaldehyde in Saccharomyces cerevisiae.
Department of Molecular Biology, Institute of Biotechnology, Vilnius, Lithuania.
A gene (FDH1) of Candida maltosa which confers resistance to formaldehyde in Saccharomyces cerevisiae was cloned and its nucleotide sequence determined. The gene has a single intron which possesses the highly conserved splicing signals found in S. cerevisiae introns. We demonstrated that processing of the pre-mRNA of the cloned gene occurred identically in both S. cerevisiae and C. maltosa. The predicted amino acid sequence from the cloned gene showed 65.5% identity to human alcohol dehydrogenase (ADH) class III and 23.9% identity to S. cerevisiae ADH1. The most probable mechanism of resistance to formaldehyde is thought to be the glutathione-dependent oxidation of formaldehyde which is characteristic for ADH class III. The cloned FDH1 gene was successfully employed as a dominant selectable marker in the transformation of S. cerevisiae.
PMID: 1339376 [PubMed - indexed for MEDLINE]
-
Cited by 5 PubMed Central articles
-
Cloning of the Arabidopsis and rice formaldehyde dehydrogenase genes: implications for the origin of plant ADH enzymes.
Dolferus R, Osterman JC, Peacock WJ, Dennis ES.
Genetics. 1997 Jul; 146(3):1131-41.
[Genetics. 1997]
-
Pea formaldehyde-active class III alcohol dehydrogenase: common derivation of the plant and animal forms but not of the corresponding ethanol-active forms (classes I and P).
Shafqat J, El-Ahmad M, Danielsson O, Martínez MC, Persson B, Parés X, Jornvall H.
Proc Natl Acad Sci U S A. 1996 May 28; 93(11):5595-9.
[Proc Natl Acad Sci U S A. 1996]
-
Characterization of a glutathione-dependent formaldehyde dehydrogenase from Rhodobacter sphaeroides.
Barber RD, Rott MA, Donohue TJ.
J Bacteriol. 1996 Mar; 178(5):1386-93.
[J Bacteriol. 1996]
- » See all...