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cDNA cloning and membrane topology of the rabbit gastric H+/K(+)-ATPase alpha-subunit.
Department of Physiology, School of Medicine, UCLA.
We have cloned and sequenced a cDNA for the rabbit gastric proton-potassium pump (H+/K(+)-ATPase) alpha-subunit. The deduced peptide contains 1035 amino acids (Mr 114,201) and shows 97% sequence identity with the respective rat and hog proteins. A monoclonal antibody 146-14 has been shown previously to react with the extracytoplasmic side of the catalytic H+/K(+)-ATPase subunit and here we show that the epitope is in the region between amino acids 855 and 902 (the numbering of the H+/K(+)-ATPase catalytic subunit throughout the paper refers to the rabbit sequence). The localization of this epitope in conjunction with previously observed trypsin cleavage sites in the C-terminal one third of the enzyme and the hydrophobicity plot of the deduced peptide sequence are evidence for a structural model for the alpha-subunit of the H+/K(+)-ATPase which contains at least ten membrane spanning segments, similar to that deduced for the Ca(2+)-ATPase of sarcoplasmic reticulum.
PMID: 1316171 [PubMed - indexed for MEDLINE]
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Cited by 2 PubMed Central articles
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Isoform-specific interactions of Na,K-ATPase subunits are mediated via extracellular domains and carbohydrates.
Schmalzing G, Ruhl K, Gloor SM.
Proc Natl Acad Sci U S A. 1997 Feb 18; 94(4):1136-41.
[Proc Natl Acad Sci U S A. 1997]
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C-terminal topology of gastric H+,K(+)-ATPase.
Asano S, Arakawa S, Hirasawa M, Sakai H, Ohta M, Ohta K, Takeguchi N.
Biochem J. 1994 Apr 1; 299 ( Pt 1):59-64.
[Biochem J. 1994]
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Potassium (Glu-K® , K+ 10® , K+ 8® , ...)
Potassium is essential for the proper functioning of the heart, kidneys, muscles, nerves, and digestive system. Usually the food you eat supplies all of the potassium you need. However, certain diseases (e.g., kidney dis...