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1: J Protein Chem. 2002 Jan;21(1):59-64.Click here to read Links

Cofactors in sarcosine oxidase from Corynebacterium sp. U-96.

Mukouyama EB, Ohsawa H, Suzuki H.

Department of Biosciences, School of Science, Kitasato University, Kanagawa, Japan. mukoyama@jet.sci.kitasato-u.ac.jp

Sarcosine oxidase from Corynebacterium sp. U-96 is a heterotetrameric enzyme that was reported to contain 1 mol of covalently bound FAD and 1 mol of non-covalently-bound FAD. This work describes the result of reinvestigation of the cofactors in this enzyme. The enzyme was found to contain 1 mol of non-covalently-bound NAD+, 1 mol of non-covalently-bound FAD, and 1 mol of covalent FMN. The covalent FMN was identified by the mass and amino acid sequence analyses of the flavin peptide.

PMID: 11902668 [PubMed - indexed for MEDLINE]