Metabolic enzymes of mycobacteria linked to antiox...[Science. 2002]

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1: Science. 2002 Feb 8;295(5557):1073-7. Epub 2002 Jan 17. Links

Metabolic enzymes of mycobacteria linked to antioxidant defense by a thioredoxin-like protein.

Bryk R, Lima CD, Erdjument-Bromage H, Tempst P, Nathan C.

Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10021, USA.

Mycobacterium tuberculosis (Mtb) mounts a stubborn defense against oxidative and nitrosative components of the immune response. Dihydrolipoamide dehydrogenase (Lpd) and dihydrolipoamide succinyltransferase (SucB) are components of alpha-ketoacid dehydrogenase complexes that are central to intermediary metabolism. We find that Lpd and SucB support Mtb's antioxidant defense. The peroxiredoxin alkyl hydroperoxide reductase (AhpC) is linked to Lpd and SucB by an adaptor protein, AhpD. The 2.0 angstrom AhpD crystal structure reveals a thioredoxin-like active site that is responsive to lipoamide. We propose that Lpd, SucB (the only lipoyl protein detected in Mtb), AhpD, and AhpC together constitute a nicotinamide adenine dinucleotide (reduced)-dependent peroxidase and peroxynitrite reductase. AhpD thus represents a class of thioredoxin-like molecules that enables an antioxidant defense.

PMID: 11799204 [PubMed - indexed for MEDLINE]

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Structures reported by this article

Structure Of Ahpd From Mycobacterium Tuberculosis, A Novel Enzyme With Thioredoxin-Like Activity

PDB: 1KNC

Source: Mycobacterium tuberculosis

Method: X-Ray Diffraction | Resolution: 2 Å

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Cited by 26 PubMed Central articles

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