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1: Science. 2001 Aug 17;293(5533):1281-5.Click here to read Links
Comment in:
Science. 2001 Aug 17;293(5533):1264-5.

Crystal structure of a carbon monoxide dehydrogenase reveals a [Ni-4Fe-5S] cluster.

Dobbek H, Svetlitchnyi V, Gremer L, Huber R, Meyer O.

Max-Planck-Institut für Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, D-82152 Martinsried, Germany. dobbek@biochem.mpg.de

The homodimeric nickel-containing CO dehydrogenase from the anaerobic bacterium Carboxydothermus hydrogenoformans catalyzes the oxidation of CO to CO2. A crystal structure of the reduced enzyme has been solved at 1.6 angstrom resolution. This structure represents the prototype for Ni-containing CO dehydrogenases from anaerobic bacteria and archaea. It contains five metal clusters of which clusters B, B', and a subunit-bridging, surface-exposed cluster D are cubane-type [4Fe-4S] clusters. The active-site clusters C and C' are novel, asymmetric [Ni-4Fe-5S] clusters. Their integral Ni ion, which is the likely site of CO oxidation, is coordinated by four sulfur ligands with square planar geometry.

PMID: 11509720 [PubMed - indexed for MEDLINE]