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1: Mol Cell. 2000 Dec;6(6):1377-87.Click here to read Links

The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases.

Yamano H, Kitamura K, Kominami K, Lehmann A, Katayama S, Hunt T, Toda T.

Laboratory of Cell Cycle Control, Imperial Cancer Research Fund, South Mimms, Herts EN6 3LD, United Kingdom.

We describe a novel set of oscillation mechanisms for the fission yeast S phase cyclin Cig2, which contains an authentic destruction box and is destroyed at anaphase via the APC/cyclosome (APC/C). Unlike the mitotic cyclin Cdc13, however, Cig2 mRNA and protein peak at the G1/S boundary and decline to low levels in G2 and M phases. We show here that SCF(Pop1, Pop2) plays a role in transcriptional periodicity, as pop mutations result in constitutive cig2(+) transcripts. The instability of Cig2 during G2 and M is independent of either the APC/C or Pop1/Pop2, but requires Skp1, a core component of SCF. These data indicate that the APC/C and SCF control Cig2 levels differentially at different stages of the cell cycle.

PMID: 11163211 [PubMed - indexed for MEDLINE]