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1: Gene. 2000 Oct 3;256(1-2):1-11.Click here to read Links

Cloning and characterization of the human ameloblastin gene.

Toyosawa S, Fujiwara T, Ooshima T, Shintani S, Sato A, Ogawa Y, Sobue S, Ijuhin N.

Department of Oral Pathology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, 565-0871, Osaka, Japan. toyosawa@dent.osaka-u.ac.jp

We isolated the full-length human ameloblastin (AMBN) cDNA clone using reverse transcription-polymerase chain reaction (RT-PCR) methods. Sequence analysis of the AMBN cDNA revealed an open reading frame of 1341bp encoding a 447-amino-acid protein. Comparison with pig, cattle, rat, and mouse AMBN sequences showed a high amino acid sequence similarity and led to the identification of a novel 78bp (26 amino acids) insert resulting from internal sequence duplication. By DNA analysis of a human genomic clones, the AMBN gene was shown to consist of 13 exons and a novel 78bp segment, which proved to comprise two small exons. Human ameloblastomas express AMBN transcripts that contain some mutations.

PMID: 11054529 [PubMed - indexed for MEDLINE]