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cDNA cloning and expression analysis of new members of the mammalian F-box protein family.
INSERM U522, Hôpital Pontchaillou, Rennes, France. Guennadi.Iline@rennes.inserm.fr
F-box proteins are critical components of the SCF ubiquitin-protein ligase complex and are involved in substrate recognition and recruitment for ubiquitination and consequent degradation by the proteasome. We have isolated cDNAs encoding a further 10 mammalian F-box proteins. Five of them (FBL3 to FBL7) share structural similarities with Skp2 and contain C-terminal leucine-rich repeats. The other 5 proteins have different putative protein-protein interaction motifs. Specifically, FBS and FBWD4 proteins contain Sec7 and WD40-repeat domains, respectively. The C-terminal region of FBA shares similarity with bacterial protein ApaG while FBG2 shows homology with the F-box protein NFB42. The marked differences in F-box gene expression in human tissues suggest their distinct role in ubiquitin-dependent protein degradation.
PMID: 10945468 [PubMed - indexed for MEDLINE]
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Cited by 8 PubMed Central articles
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PML activates transcription by protecting HIPK2 and p300 from SCFFbx3-mediated degradation.
Shima Y, Shima T, Chiba T, Irimura T, Pandolfi PP, Kitabayashi I.
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[Mol Cell Biol. 2008]
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Yano H, Kobayashi I, Onodera Y, Luton F, Franco M, Mazaki Y, Hashimoto S, Iwai K, Ronai Z, Sabe H.
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[Mol Biol Cell. 2008]
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Preparation, crystallization and preliminary X-ray analysis of XC2382, an ApaG protein of unknown structure from Xanthomonas campestris.
Chin KH, Chou CC, Lee CC, Shr HL, Lyu PC, Wang AH, Chou SH.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1; 61(Pt 7):700-2. Epub 2005 Jun 30.
[Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005]
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