Routine HLA-B genotyping with PCR-sequence-specific oligonucleotides (PCR-SSO) detects eight new alleles: B*0807, B*0809, B*1551, B*3529, B*3532, B*4025, B*5304 and B*5508.
Kennedy CT,
Dodd R,
Le T,
Wallace R,
Ng G,
Greville WD,
Kennedy A,
Taverniti A,
Moses JH,
Clow N,
Watson N,
Dunckley H.
Molecular Genetics Laboratory, Tissue Typing, Australian Red Cross Blood Service, Sydney, New South Wales, Australia. ckennedy@arcbs.redcross.org.au
This paper describes eight new alleles (B*0807, B*0809, B*1551, B*3529, B*3532, B*4025, B*5304 and B*5508) that have been found by routine HLA-B genotyping with sequence-specific oligonucleotides (SSOs). All of the new alleles have variations which cause changes in residues that occur within antigen binding pockets and T-cell recognition sites of the antigen. The new polymorphisms within these new alleles may affect the nature and specificity of peptide binding and cause differential T-cell activation, which may have an affect in transplantation.
PMID: 10777103 [PubMed - indexed for MEDLINE]