-
Nuclear localization of the Arabidopsis blue light receptor cryptochrome 2.
FB Biologie/Botanik, Philipps-Universität, Marburg, Germany.
The cryptochrome blue light photoreceptor family of Arabidopsis thaliana consists of two members, CRY1 and CRY2 (PHH1). CRY2 contains a putative nuclear localization signal (NLS) within its C-terminal region. We examined whether CRY2 is localized in the nucleus and whether the C-terminal region of CRY2 is involved in nuclear targeting. Total cellular and nuclear protein extracts from Arabidopsis were subjected to immunoblot analysis with CRY2-specific antibodies. Strong CRY2 signals were obtained in the nuclear fraction. Fusion proteins consisting of the green fluorescent protein (GFP) and different fragments of CRY2 were expressed in parsley protoplasts and the localization of the fusion proteins was determined by fluorescence and confocal laser scanning microscopy. GFP-fusions containing the entire CRY2 protein or its C-terminal region were found exclusively in the nucleus. We conclude from these results that CRY2 is localized in the nucleus and that nuclear localization is mediated by the C-terminal region of CRY2.
PMID: 10476076 [PubMed - indexed for MEDLINE]
-
Cited by 14 PubMed Central articles
-
Wheat cryptochromes: subcellular localization and involvement in photomorphogenesis and osmotic stress responses.
Xu P, Xiang Y, Zhu H, Xu H, Zhang Z, Zhang C, Zhang L, Ma Z.
Plant Physiol. 2009 Feb; 149(2):760-74. Epub 2008 Dec 3.
[Plant Physiol. 2009]
-
HFR1 is crucial for transcriptome regulation in the cryptochrome 1-mediated early response to blue light in Arabidopsis thaliana.
Zhang XN, Wu Y, Tobias JW, Brunk BP, Deitzer GF, Liu D.
PLoS One. 2008; 3(10):e3563. Epub 2008 Oct 30.
[PLoS One. 2008]
-
Separate functions for nuclear and cytoplasmic cryptochrome 1 during photomorphogenesis of Arabidopsis seedlings.
Wu G, Spalding EP.
Proc Natl Acad Sci U S A. 2007 Nov 20; 104(47):18813-8. Epub 2007 Nov 14.
[Proc Natl Acad Sci U S A. 2007]
- » See all...