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1: FEBS Lett. 1999 Mar 19;447(1):39-43.Click here to read Links

Demonstration of the pathogenic effect of point mutated keratin 9 in vivo.

Kobayashi S, Kore-eda S, Tanaka T.

Department of Dermatology, Graduate School of Medicine, Kyoto University, Japan.

A wild type keratin 9 (K9) cDNA and a point mutated keratin 9 cDNA were injected subcutaneously into mouse skin. The hemagglutinin tag staining of the wild type K9 cDNA injected specimens mainly showed a homogeneous pattern, whereas the point mutated K9 cDNA injected specimens mainly showed a granular pattern in the suprabasal cells. Double staining of K9 and the endogenous keratin revealed the incorporation of de novo synthesized K9 into the keratin network. These results demonstrate that (1) a naked DNA transfection into mouse skin can detect the pathogenic changes of point mutated keratin in vivo and (2) the keratin 9 mutation disrupts the keratin network formation in the suprabasal cells in vivo.

PMID: 10218578 [PubMed - indexed for MEDLINE]