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2000 10
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786 results

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Page 1
Small-scale expression of proteins in E. coli.
Zerbs S, Giuliani S, Collart F. Zerbs S, et al. Methods Enzymol. 2014;536:117-31. doi: 10.1016/B978-0-12-420070-8.00011-8. Methods Enzymol. 2014. PMID: 24423272
The subcloning process also provides an opportunity to introduce purification tags, epitope tags, fusions, truncations, and mutations into the coding sequence that may be useful in downstream purification or characterization applications. ...Small-scale expression c …
The subcloning process also provides an opportunity to introduce purification tags, epitope tags, fusions, truncations, and mu …
Making the most of affinity tags.
Waugh DS. Waugh DS. Trends Biotechnol. 2005 Jun;23(6):316-20. doi: 10.1016/j.tibtech.2005.03.012. Trends Biotechnol. 2005. PMID: 15922084 Review.
Proteins do not naturally lend themselves to high-throughput analysis because of their diverse physiochemical properties. Consequently, affinity tags have become indispensable tools for structural and functional proteomics initiatives. ...However, no single affinity tag is …
Proteins do not naturally lend themselves to high-throughput analysis because of their diverse physiochemical properties. Consequently, affi …
Design and applications of lanthanide chelating tags for pseudocontact shift NMR spectroscopy with biomacromolecules.
Joss D, Häussinger D. Joss D, et al. Prog Nucl Magn Reson Spectrosc. 2019 Oct-Dec;114-115:284-312. doi: 10.1016/j.pnmrs.2019.08.002. Epub 2019 Aug 28. Prog Nucl Magn Reson Spectrosc. 2019. PMID: 31779884 Review.
Furthermore, all DOTA-derived lanthanide chelating tags used for PCS NMR spectroscopy published to date are displayed in tabular form, including their anisotropy parameters, with all employed lanthanide ions, C(B)-Ln distances and tagging reaction conditions, i.e., …
Furthermore, all DOTA-derived lanthanide chelating tags used for PCS NMR spectroscopy published to date are displayed in tabular form …
Gap-enhanced Raman tags: fabrication, optical properties, and theranostic applications.
Khlebtsov NG, Lin L, Khlebtsov BN, Ye J. Khlebtsov NG, et al. Theranostics. 2020 Jan 12;10(5):2067-2094. doi: 10.7150/thno.39968. eCollection 2020. Theranostics. 2020. PMID: 32089735 Free PMC article. Review.
Gap-enhanced Raman tags (GERTs) are emerging probes of surface-enhanced Raman scattering (SERS) spectroscopy that have found promising analytical, bioimaging, and theranostic applications. ...Our discussion is focused on the design and synthetic strategies that ensure the …
Gap-enhanced Raman tags (GERTs) are emerging probes of surface-enhanced Raman scattering (SERS) spectroscopy that have found promisin …
Transparent UHF RFID tags based on CVD-grown graphene films.
Yin M, Wu L, Chen H, Zhang X, Wang W, Liu Z. Yin M, et al. Nanotechnology. 2022 Oct 3;33(50). doi: 10.1088/1361-6528/ac8e74. Nanotechnology. 2022. PMID: 36049467
Through structural design, parameter optimization and experimental measurements, the reading distance of the transparent RFID tags was tested and compared. ...In addition, the reading distance of graphene-based RFID tags at different bending angles and cycles …
Through structural design, parameter optimization and experimental measurements, the reading distance of the transparent RFID tags …
Polymeric dipicolylamine based mass tags for mass cytometry.
Zhang Y, Liu P, Majonis D, Winnik MA. Zhang Y, et al. Chem Sci. 2022 Feb 23;13(11):3233-3243. doi: 10.1039/d2sc00595f. eCollection 2022 Mar 16. Chem Sci. 2022. PMID: 35414868 Free PMC article.
While there are more than 100 stable isotopes available in the m/z 75 to 209 detection range of the instrument, only about 50 parameters can be measured per cell because current reagents are metal-chelating polymers with pendant aminocarboxylate chelators that only bind ha …
While there are more than 100 stable isotopes available in the m/z 75 to 209 detection range of the instrument, only about 50 parameters …
Tag-based spite with correlated interactions.
Bruner JP, Smead R. Bruner JP, et al. J Theor Biol. 2022 May 7;540:111052. doi: 10.1016/j.jtbi.2022.111052. Epub 2022 Mar 2. J Theor Biol. 2022. PMID: 35247376
The presence of tags shows that pure spite is less likely to evolve when theoretically expected, but that conditional spite is more likely than expected. ...We discuss implications for the evolution of spite, punishment, and the methodological approach of using exogenous …
The presence of tags shows that pure spite is less likely to evolve when theoretically expected, but that conditional spite is more l …
Exploiting Molecular Barcodes in High-Throughput Cellular Assays.
Binan L, Drobetsky EA, Costantino S. Binan L, et al. SLAS Technol. 2019 Jun;24(3):298-307. doi: 10.1177/2472630318824337. Epub 2019 Feb 1. SLAS Technol. 2019. PMID: 30707854 Review.
Multiplexing strategies, which greatly increase the number of simultaneously measured parameters in single experiments, are now being widely implemented by both the pharmaceutical industry and academic researchers. ...Here we review the basic principles of several technolo …
Multiplexing strategies, which greatly increase the number of simultaneously measured parameters in single experiments, are now being …
Ionic polypeptide tags for protein phase separation.
Kapelner RA, Obermeyer AC. Kapelner RA, et al. Chem Sci. 2019 Jan 17;10(9):2700-2707. doi: 10.1039/c8sc04253e. eCollection 2019 Mar 7. Chem Sci. 2019. PMID: 30996987 Free PMC article.
Recent work indicates that proteins with an intrinsically disordered region, a higher net charge, or a patch of charged residues are more likely to undergo a phase transition. Based on these design parameters, polyionic coacervation tags were designed and assessed f …
Recent work indicates that proteins with an intrinsically disordered region, a higher net charge, or a patch of charged residues are more li …
Elucidating host-microbe interactions in vivo by studying population dynamics using neutral genetic tags.
Hausmann A, Hardt WD. Hausmann A, et al. Immunology. 2021 Apr;162(4):341-356. doi: 10.1111/imm.13266. Epub 2020 Oct 19. Immunology. 2021. PMID: 32931019 Free PMC article. Review.
Recent approaches - combining experiments using neutral genetic tags with stochastic population dynamic models - allow more precise quantification of biologically relevant parameters that govern the interaction between microbe and host cell populations. ...In this r …
Recent approaches - combining experiments using neutral genetic tags with stochastic population dynamic models - allow more precise q …
786 results