Multiple approaches for massively parallel sequencing of SARS-CoV-2 genomes directly from clinical samples.
Xiao M, Liu X, Ji J, Li M, Li J, Yang L, Sun W, Ren P, Yang G, Zhao J, Liang T, Ren H, Chen T, Zhong H, Song W, Wang Y, Deng Z, Zhao Y, Ou Z, Wang D, Cai J, Cheng X, Feng T, Wu H, Gong Y, Yang H, Wang J, Xu X, Zhu S, Chen F, Zhang Y, Chen W, Li Y, Li J.
Xiao M, et al.
Genome Med. 2020 Jun 30;12(1):57. doi: 10.1186/s13073-020-00751-4.
Genome Med. 2020.
PMID: 32605661
Free PMC article.
RESULTS: We demonstrated that both amplicon and capture methods efficiently enriched SARS-CoV-2 content from clinical samples, while the enrichment efficiency of amplicon outran that of capture in more challenging samples. We found that capture was not as accurate as meta …
RESULTS: We demonstrated that both amplicon and capture methods efficiently enriched SARS-CoV-2 content from clinical samples, while the enr …