Items per page
Sort by

Send to:

Choose Destination

Search results

Items: 19


A putative Type IIS restriction endonuclease GeoICI from Geobacillus sp. - A robust, thermostable alternative to mezophilic prototype BbvI.

Zebrowska J, Zolnierkiewicz O, Skowron MA, Zylicz-Stachula A, Jezewska-Frackowiak J, Skowron PM.

J Biosci. 2016 Mar;41(1):27-38.


Engineering TaqII bifunctional endonuclease DNA recognition fidelity: the effect of a single amino acid substitution within the methyltransferase catalytic site.

Zylicz-Stachula A, Zebrowska J, Czajkowska E, Wrese W, Sulecka E, Skowron PM.

Mol Biol Rep. 2016 Apr;43(4):269-82. doi: 10.1007/s11033-016-3949-3. Epub 2016 Feb 17.


Two-stage gene assembly/cloning of a member of the TspDTI subfamily of bifunctional restriction endonucleases, TthHB27I.

Krefft D, Zylicz-Stachula A, Mulkiewicz E, Papkov A, Jezewska-Frackowiak J, Skowron PM.

J Biotechnol. 2015 Jan 20;194:67-80. doi: 10.1016/j.jbiotec.2014.11.030. Epub 2014 Dec 6.


A new prototype IIS/IIC/IIG endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus, recognising 5'-TARCCA(N11/9)-3' sequences.

Jezewska-Frackowiak J, Lubys A, Vitkute J, Zakareviciene L, Zebrowska J, Krefft D, Skowron MA, Zylicz-Stachula A, Skowron PM.

J Biotechnol. 2015 Jan 20;194:19-26. doi: 10.1016/j.jbiotec.2014.11.023. Epub 2014 Dec 4.


Artificial plasmid labeled with 5-bromo-2'-deoxyuridine: a universal molecular system for strand break detection.

Zylicz-Stachula A, Polska K, Skowron P, Rak J.

Chembiochem. 2014 Jul 7;15(10):1409-12. doi: 10.1002/cbic.201402082. Epub 2014 May 21.


Photoinduced single strand breaks and intrastrand cross-links in an oligonucleotide labeled with 5-bromouracil.

Zdrowowicz M, Michalska B, Zylicz-Stachula A, Rak J.

J Phys Chem B. 2014 May 15;118(19):5009-16. doi: 10.1021/jp500192z. Epub 2014 May 2.


Cofactor analogue-induced chemical reactivation of endonuclease activity in a DNA cleavage/methylation deficient TspGWI N₄₇₃A variant in the NPPY motif.

Zylicz-Stachula A, Jeżewska-Frąckowiak J, Skowron PM.

Mol Biol Rep. 2014;41(4):2313-23. doi: 10.1007/s11033-014-3085-x. Epub 2014 Jan 19.


Modified 'one amino acid-one codon' engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase.

Zylicz-Stachula A, Zolnierkiewicz O, Sliwinska K, Jezewska-Frackowiak J, Skowron PM.

Microb Cell Fact. 2014 Jan 11;13:7. doi: 10.1186/1475-2859-13-7.


Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus.

Skowron PM, Vitkute J, Ramanauskaite D, Mitkaite G, Jezewska-Frackowiak J, Zebrowska J, Zylicz-Stachula A, Lubys A.

BMC Mol Biol. 2013 Aug 6;14:17. doi: 10.1186/1471-2199-14-17.


A new genomic tool, ultra-frequently cleaving TaqII/sinefungin endonuclease with a combined 2.9-bp recognition site, applied to the construction of horse DNA libraries.

Zylicz-Stachula A, Zolnierkiewicz O, Jasiecki J, Skowron PM.

BMC Genomics. 2013 Jun 1;14:370. doi: 10.1186/1471-2164-14-370.


Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities.

Zylicz-Stachula A, Zolnierkiewicz O, Lubys A, Ramanauskaite D, Mitkaite G, Bujnicki JM, Skowron PM.

BMC Mol Biol. 2012 Apr 10;13:13. doi: 10.1186/1471-2199-13-13.


Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving.

Zylicz-Stachula A, Zołnierkiewicz O, Sliwińska K, Jeżewska-Frąckowiak J, Skowron PM.

BMC Biochem. 2011 Dec 5;12:62. doi: 10.1186/1471-2091-12-62.


Enzymatic synthesis of long double-stranded DNA labeled with haloderivatives of nucleobases in a precisely pre-determined sequence.

Sobolewski I, Polska K, Zylicz-Stachula A, Jeżewska-Frąckowiak J, Rak J, Skowron P.

BMC Biochem. 2011 Aug 24;12:47. doi: 10.1186/1471-2091-12-47.


PCR synthesis of double stranded DNA labeled with 5-bromouridine. A step towards finding a bromonucleoside for clinical trials.

Michalska B, Sobolewski I, Polska K, Zielonka J, Zylicz-Stachula A, Skowron P, Rak J.

J Pharm Biomed Anal. 2011 Dec 5;56(4):671-7. doi: 10.1016/j.jpba.2011.07.036. Epub 2011 Jul 30.


Chemically-induced affinity star restriction specificity: a novel TspGWI/sinefungin endonuclease with theoretical 3-bp cleavage frequency.

Zylicz-Stachula A, Zołnierkiewicz O, Jeżewska-Frąckowiak J, Skowron PM.

Biotechniques. 2011 Jun;50(6):397-406. doi: 10.2144/000113685.


Unexpected photoproduct generated via the acetone-sensitized photolysis of 5-bromo-2'-deoxyuridine in a water/isopropanol solution: experimental and computational studies.

Polska K, Zielonka J, Chomicz L, Czerwicka M, Stepnowski P, Guzow K, Wiczk W, Smużyńska M, Kasprzykowski F, Żylicz-Stachula A, Skowron P, Rak J.

J Phys Chem B. 2010 Dec 23;114(50):16902-7. doi: 10.1021/jp1071499. Epub 2010 Nov 30.


Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family.

Zylicz-Stachula A, Bujnicki JM, Skowron PM.

BMC Mol Biol. 2009 May 29;10:52. doi: 10.1186/1471-2199-10-52. Erratum in: BMC Mol Biol. 2014;15:16.


A new Thermus sp. class-IIS enzyme sub-family: isolation of a 'twin' endonuclease TspDTI with a novel specificity 5'-ATGAA(N(11/9))-3', related to TspGWI, TaqII and Tth111II.

Skowron PM, Majewski J, Zylicz-Stachula A, Rutkowska SM, Jaworowska I, Harasimowicz-Słowińska RI.

Nucleic Acids Res. 2003 Jul 15;31(14):e74.


TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5'-ACGGA(N11/9)-3'.

Zylicz-Stachula A, Harasimowicz-Slowińska RI, Sobolewski I, Skowron PM.

Nucleic Acids Res. 2002 Apr 1;30(7):e33.

Items per page
Sort by

Send to:

Choose Destination

Supplemental Content

Loading ...
Write to the Help Desk