Science. 1995 Jul 28;269(5223):496-512.

Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.

Fleischmann RD, Adams MD, White O, Clayton RA, Kirkness EF, Kerlavage AR, Bult CJ, Tomb JF, Dougherty BA, Merrick JM, et al.

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Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Abstract

An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.

Comment in

Bacterial genome sequence bagged. [Science. 1995]
Bacterial genome sequence bagged.Nowak R. Science. 1995 Jul 28; 269(5223):468-70.
Pioneering work. [Science. 1995]
Pioneering work.Fiers W. Science. 1995 Sep 29; 269(5232):1805.
More Haemophilus and Mycoplasma genes. [Science. 1996]
More Haemophilus and Mycoplasma genes.Robison K, Gilbert W, Church GM. Science. 1996 Mar 1; 271(5253):1302-3; author reply 1303-4.
More Haemophilus and Mycoplasma genes. [Science. 1996]
More Haemophilus and Mycoplasma genes.Brosius J. Science. 1996 Mar 1; 271(5253):1302; author reply 1303-4.

PMID:: 7542800; [PubMed - indexed for MEDLINE]

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Assembly
Assembly
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
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Substance (MeSH Keyword)
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Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Genome
Genome records that include the current articles as references. These are typically the articles that report the sequencing and analysis of the genome.
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Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.
Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.
Science. 1995 Jul 28 ;269(5223):496-512.

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