FIG. 1.

PCR products amplified from negative (water) controls with primers targeting the bla_TEM gene. The product was run on a 1% agarose gel with 0.5× Tris-acetate-EDTA buffer. All reactions were performed in duplicate. Lane M, DNA size marker; lanes 1 and 2, PCR buffer from manufacturer B with Taq DNA polymerase from manufacturer B; lanes 3 and 4, PCR buffer from manufacturer B with Taq DNA polymerase from manufacturer A; lanes 5 and 6, PCR buffer from manufacturer A with Taq DNA polymerase from manufacturer B; lanes 7 and 8, PCR buffer from manufacturer A with Taq DNA polymerase from manufacturer A. The arrow indicates the amplified bla_TEM-1 gene fragment.

Presence of ?-Lactamase Gene TEM-1 DNA Sequence in Commercial Taq DNA Polymerase

J Clin Microbiol. 2005 January;43(1):530-531.
FIG. 8

Activity of FEN on modified pseudo-Y substrates at 50°C. (A) Lanes: 1, marker (FS, 37 nt); 2 and 3, FS after incubation of FS plus FA with Taq DNA polymerase and FEN, respectively; 4 and 5, FS after incubation of FS plus FA-T with Taq DNA polymerase and FEN, respectively (see Fig. 2C); 6 and 7, FS after incubation of FS, FA, and FA-C (hybridized to FA) with Taq DNA polymerase and FEN, respectively (see Fig. 2D). (B) Lanes: 1, 21-nt marker; 2, oligonucleotide FS (37 nt); 3 and 5, FS after incubation of FS plus FA (unmodified substrate) with Taq DNA polymerase and FEN, respectively; 4 and 6, FS after incubation of FS, FA, and FS-C (hybridized to FS) with Taq DNA polymerase and FEN, respectively (see Fig. 2E). (C) Lanes: 1, marker formed by ligating oligonucleotide FS-L to ³²P-labeled FS to form a hairpin loop (see Fig. 2F); 2 to 4, hairpin loop after incubation with Taq DNA polymerase for 5, 15, and 45 min, respectively; 5 to 7, hairpin loop after incubation with FEN for 5, 15, and 45 min, respectively.

Methanococcus jannaschii Flap Endonuclease: Expression, Purification, and Substrate Requirements

J Bacteriol. 1998 October;180(20):5406-5412.
Figure 3

Identification of the recombinant Taq DNA polymerase enzyme by Western blotting using anti-His antibody. Lane 1: Control, expressed pET15b plasmid in E. coli BL21 cells. Lane 2: Control, E. coli BL21 (DE3) crude lysate. Lane 3: Protein molecular weight marker Lanes 4, 5, and 6: Expressed and purified Taq DNA polymerase in BL21 (DE3) bacteria. Arrow shows 94kDa Taq DNA polymerase band

Optimization of Taq DNA polymerase enzyme expression in Escherichia coli

Adv Biomed Res. 2012;1:82.
Figure 1

Superimposition of the thumb domains of Taq DNA polymerase (blue) with T7 DNA polymerase (pink). The arrows indicate the site of insertion of the T3 TBD (yellow). The primary amino acid sequence of Taq DNA polymerase from residue 470–507 is indicated below (blue) with the sequence of T3 TBD in yellow and the deleted region in red.

Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase

Nucleic Acids Res. 2003 August 15;31(16):4702-4709.

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Structure of Taq polymerase with DNA at the polymerase active site. [Nature. 1996]
Structure of Taq polymerase with DNA at the polymerase active site.
Eom SH, Wang J, Steitz TA. Nature. 1996 Jul 18; 382(6588):278-81.
Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. [Biotechniques. 1996]
Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping.
Brownstein MJ, Carpten JD, Smith JR. Biotechniques. 1996 Jun; 20(6):1004-6, 1008-10.
Production and evaluation of Taq DNA polymerase. [J Med Assoc Thai. 1997]
Production and evaluation of Taq DNA polymerase.
Leelayuwat C, Srisuk T, Paechaiyaphum R, Limpaiboon T, Romphruk A, Romphruk A. J Med Assoc Thai. 1997 Sep; 80 Suppl 1:S129-37.

590 free full-text articles in PubMed Central

Optimization of Taq DNA polymerase enzyme expression in Escherichia coli. [Adv Biomed Res. 2012]
Optimization of Taq DNA polymerase enzyme expression in Escherichia coli.
Moazen F, Rastegari A, Hoseini SM, Panjehpour M, Miroliaei M, Sadeghi HM. Adv Biomed Res. 2012; 1:82. Epub 2012 Oct 31.
Pinot blanc and Pinot gris arose as independent somatic mutations of Pinot noir. [J Exp Bot. 2012]
Pinot blanc and Pinot gris arose as independent somatic mutations of Pinot noir.
Vezzulli S, Leonardelli L, Malossini U, Stefanini M, Velasco R, Moser C. J Exp Bot. 2012 Nov; 63(18):6359-69. Epub 2012 Oct 23.
RNA polymerase-promoter interactions determining different stability of the Escherichia coli and Thermus aquaticus transcription initiation complexes. [Nucleic Acids Res. 2012]
RNA polymerase-promoter interactions determining different stability of the Escherichia coli and Thermus aquaticus transcription initiation complexes.
Mekler V, Minakhin L, Kuznedelov K, Mukhamedyarov D, Severinov K. Nucleic Acids Res. 2012 Dec; 40(22):11352-62. Epub 2012 Oct 18.