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A putative Type IIS restriction endonuclease GeoICI from Geobacillus sp.--A robust, thermostable alternative to mezophilic prototype BbvI.

Zebrowska J, Zolnierkiewicz O, Skowron MA, Zylicz-Stachula A, Jezewska-Frackowiak J, Skowron PM.

J Biosci. 2016 Mar;41(1):27-38.


Engineering TaqII bifunctional endonuclease DNA recognition fidelity: the effect of a single amino acid substitution within the methyltransferase catalytic site.

Zylicz-Stachula A, Zebrowska J, Czajkowska E, Wrese W, Sulecka E, Skowron PM.

Mol Biol Rep. 2016 Apr;43(4):269-82. doi: 10.1007/s11033-016-3949-3. Epub 2016 Feb 17.


MmoSTI restriction endonuclease, isolated from Morganella morganii infecting a tropical moth, Actias selene, cleaving 5'-|CCNGG-3' sequences.

Skowron MA, Zebrowska J, Wegrzyn G, Skowron PM.

J Appl Genet. 2016 Feb;57(1):143-9. doi: 10.1007/s13353-015-0308-3. Epub 2015 Aug 18.


Two-stage gene assembly/cloning of a member of the TspDTI subfamily of bifunctional restriction endonucleases, TthHB27I.

Krefft D, Zylicz-Stachula A, Mulkiewicz E, Papkov A, Jezewska-Frackowiak J, Skowron PM.

J Biotechnol. 2015 Jan 20;194:67-80. doi: 10.1016/j.jbiotec.2014.11.030. Epub 2014 Dec 6.


A new prototype IIS/IIC/IIG endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus, recognising 5'-TARCCA(N11/9)-3' sequences.

Jezewska-Frackowiak J, Lubys A, Vitkute J, Zakareviciene L, Zebrowska J, Krefft D, Skowron MA, Zylicz-Stachula A, Skowron PM.

J Biotechnol. 2015 Jan 20;194:19-26. doi: 10.1016/j.jbiotec.2014.11.023. Epub 2014 Dec 4.


Cofactor analogue-induced chemical reactivation of endonuclease activity in a DNA cleavage/methylation deficient TspGWI N₄₇₃A variant in the NPPY motif.

Zylicz-Stachula A, Jeżewska-Frąckowiak J, Skowron PM.

Mol Biol Rep. 2014;41(4):2313-23. doi: 10.1007/s11033-014-3085-x. Epub 2014 Jan 19.


Modified 'one amino acid-one codon' engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase.

Zylicz-Stachula A, Zolnierkiewicz O, Sliwinska K, Jezewska-Frackowiak J, Skowron PM.

Microb Cell Fact. 2014 Jan 11;13:7. doi: 10.1186/1475-2859-13-7.


Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus.

Skowron PM, Vitkute J, Ramanauskaite D, Mitkaite G, Jezewska-Frackowiak J, Zebrowska J, Zylicz-Stachula A, Lubys A.

BMC Mol Biol. 2013 Aug 6;14:17. doi: 10.1186/1471-2199-14-17.


A new genomic tool, ultra-frequently cleaving TaqII/sinefungin endonuclease with a combined 2.9-bp recognition site, applied to the construction of horse DNA libraries.

Zylicz-Stachula A, Zolnierkiewicz O, Jasiecki J, Skowron PM.

BMC Genomics. 2013 Jun 1;14:370. doi: 10.1186/1471-2164-14-370.


Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities.

Zylicz-Stachula A, Zolnierkiewicz O, Lubys A, Ramanauskaite D, Mitkaite G, Bujnicki JM, Skowron PM.

BMC Mol Biol. 2012 Apr 10;13:13. doi: 10.1186/1471-2199-13-13.


Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving.

Zylicz-Stachula A, Zołnierkiewicz O, Sliwińska K, Jeżewska-Frąckowiak J, Skowron PM.

BMC Biochem. 2011 Dec 5;12:62. doi: 10.1186/1471-2091-12-62.


Chemically-induced affinity star restriction specificity: a novel TspGWI/sinefungin endonuclease with theoretical 3-bp cleavage frequency.

Zylicz-Stachula A, Zołnierkiewicz O, Jeżewska-Frąckowiak J, Skowron PM.

Biotechniques. 2011 Jun;50(6):397-406. doi: 10.2144/000113685.


Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family.

Zylicz-Stachula A, Bujnicki JM, Skowron PM.

BMC Mol Biol. 2009 May 29;10:52. doi: 10.1186/1471-2199-10-52. Erratum in: BMC Mol Biol. 2014;15:16.


A new Thermus sp. class-IIS enzyme sub-family: isolation of a 'twin' endonuclease TspDTI with a novel specificity 5'-ATGAA(N(11/9))-3', related to TspGWI, TaqII and Tth111II.

Skowron PM, Majewski J, Zylicz-Stachula A, Rutkowska SM, Jaworowska I, Harasimowicz-Słowińska RI.

Nucleic Acids Res. 2003 Jul 15;31(14):e74.


TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5'-ACGGA(N11/9)-3'.

Zylicz-Stachula A, Harasimowicz-Slowińska RI, Sobolewski I, Skowron PM.

Nucleic Acids Res. 2002 Apr 1;30(7):e33.


Rapid screening of elution conditions prior to immunopurification of proteins.

Rutkowska SM, Skowron PM.

Biotechniques. 1999 Nov;27(5):930-2, 934. No abstract available.


Sequential amplification of cloned DNA as tandem multimers using class-IIS restriction enzymes.

Lee JH, Skowron PM, Rutkowska SM, Hong SS, Kim SC.

Genet Anal. 1996 Dec;13(6):139-45.


Molecular cloning of the three base restriction endonuclease R.CviJI from eukaryotic Chlorella virus IL-3A.

Swaminathan N, Mead DA, McMaster K, George D, Van Etten JL, Skowron PM.

Nucleic Acids Res. 1996 Jul 1;24(13):2463-9.


Structural requirements for FokI-DNA interaction and oligodeoxyribonucleotide-instructed cleavage.

Kim SC, Skowron PM, Szybalski W.

J Mol Biol. 1996 May 17;258(4):638-49.

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