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Results: 1 to 20 of 21

1.

Cofactor analogue-induced chemical reactivation of endonuclease activity in a DNA cleavage/methylation deficient TspGWI N₄₇₃A variant in the NPPY motif.

Zylicz-Stachula A, Jeżewska-Frąckowiak J, Skowron PM.

Mol Biol Rep. 2014 Apr;41(4):2313-23. doi: 10.1007/s11033-014-3085-x. Epub 2014 Jan 19.

PMID:
24442320
[PubMed - in process]
Free PMC Article
2.

Modified 'one amino acid-one codon' engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase.

Zylicz-Stachula A, Zolnierkiewicz O, Sliwinska K, Jezewska-Frackowiak J, Skowron PM.

Microb Cell Fact. 2014 Jan 11;13:7. doi: 10.1186/1475-2859-13-7.

PMID:
24410856
[PubMed - in process]
Free PMC Article
3.

Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus.

Skowron PM, Vitkute J, Ramanauskaite D, Mitkaite G, Jezewska-Frackowiak J, Zebrowska J, Zylicz-Stachula A, Lubys A.

BMC Mol Biol. 2013 Aug 6;14:17. doi: 10.1186/1471-2199-14-17.

PMID:
23919831
[PubMed - indexed for MEDLINE]
Free PMC Article
4.

A new genomic tool, ultra-frequently cleaving TaqII/sinefungin endonuclease with a combined 2.9-bp recognition site, applied to the construction of horse DNA libraries.

Zylicz-Stachula A, Zolnierkiewicz O, Jasiecki J, Skowron PM.

BMC Genomics. 2013 Jun 1;14(1):370. doi: 10.1186/1471-2164-14-370.

PMID:
23724933
[PubMed - in process]
Free PMC Article
5.

Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities.

Zylicz-Stachula A, Zolnierkiewicz O, Lubys A, Ramanauskaite D, Mitkaite G, Bujnicki JM, Skowron PM.

BMC Mol Biol. 2012 Apr 10;13:13. doi: 10.1186/1471-2199-13-13.

PMID:
22489904
[PubMed - indexed for MEDLINE]
Free PMC Article
6.

Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving.

Zylicz-Stachula A, Zołnierkiewicz O, Sliwińska K, Jeżewska-Frąckowiak J, Skowron PM.

BMC Biochem. 2011 Dec 5;12:62. doi: 10.1186/1471-2091-12-62.

PMID:
22141927
[PubMed - indexed for MEDLINE]
Free PMC Article
7.

Chemically-induced affinity star restriction specificity: a novel TspGWI/sinefungin endonuclease with theoretical 3-bp cleavage frequency.

Zylicz-Stachula A, Zołnierkiewicz O, Jeżewska-Frąckowiak J, Skowron PM.

Biotechniques. 2011 Jun;50(6):397-406. doi: 10.2144/000113685.

PMID:
21781040
[PubMed - indexed for MEDLINE]
Free Article
8.

Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family.

Zylicz-Stachula A, Bujnicki JM, Skowron PM.

BMC Mol Biol. 2009 May 29;10:52. doi: 10.1186/1471-2199-10-52.

PMID:
19480701
[PubMed - indexed for MEDLINE]
Free PMC Article
9.

A new Thermus sp. class-IIS enzyme sub-family: isolation of a 'twin' endonuclease TspDTI with a novel specificity 5'-ATGAA(N(11/9))-3', related to TspGWI, TaqII and Tth111II.

Skowron PM, Majewski J, Zylicz-Stachula A, Rutkowska SM, Jaworowska I, Harasimowicz-Słowińska RI.

Nucleic Acids Res. 2003 Jul 15;31(14):e74.

PMID:
12853651
[PubMed - indexed for MEDLINE]
Free PMC Article
10.

TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5'-ACGGA(N11/9)-3'.

Zylicz-Stachula A, Harasimowicz-Slowińska RI, Sobolewski I, Skowron PM.

Nucleic Acids Res. 2002 Apr 1;30(7):e33.

PMID:
11917039
[PubMed - indexed for MEDLINE]
Free PMC Article
11.

Rapid screening of elution conditions prior to immunopurification of proteins.

Rutkowska SM, Skowron PM.

Biotechniques. 1999 Nov;27(5):930-2, 934. No abstract available.

PMID:
10572639
[PubMed - indexed for MEDLINE]
12.

Thermal cycle labeling: zeptomole detection sensitivity and microgram probe amplification using CviJl* restriction-generated oligonucleotides.

Swaminathan N, McMaster K, Skowron PM, Mead DA.

Anal Biochem. 1998 Jan 1;255(1):133-41.

PMID:
9448852
[PubMed - indexed for MEDLINE]
13.

Sequential amplification of cloned DNA as tandem multimers using class-IIS restriction enzymes.

Lee JH, Skowron PM, Rutkowska SM, Hong SS, Kim SC.

Genet Anal. 1996 Dec;13(6):139-45.

PMID:
9117889
[PubMed - indexed for MEDLINE]
14.

Molecular cloning of the three base restriction endonuclease R.CviJI from eukaryotic Chlorella virus IL-3A.

Swaminathan N, Mead DA, McMaster K, George D, Van Etten JL, Skowron PM.

Nucleic Acids Res. 1996 Jul 1;24(13):2463-9.

PMID:
8692682
[PubMed - indexed for MEDLINE]
Free PMC Article
15.

Structural requirements for FokI-DNA interaction and oligodeoxyribonucleotide-instructed cleavage.

Kim SC, Skowron PM, Szybalski W.

J Mol Biol. 1996 May 17;258(4):638-49.

PMID:
8636998
[PubMed - indexed for MEDLINE]
16.

GCN4 eukaryotic transcription factor/FokI endonuclease-mediated 'Achilles' heel cleavage': quantitative study of protein-DNA interaction.

Skowron PM, Harasimowicz R, Rutkowska SM.

Gene. 1996 Apr 17;170(1):1-8.

PMID:
8621067
[PubMed - indexed for MEDLINE]
17.

MmeI, a class-IIS restriction endonuclease: purification and characterization.

Tucholski J, Skowron PM, Podhajska AJ.

Gene. 1995 May 19;157(1-2):87-92.

PMID:
7607532
[PubMed - indexed for MEDLINE]
18.

Cloning and applications of the two/three-base restriction endonuclease R.CviJI from IL-3A virus-infected Chlorella.

Skowron PM, Swaminathan N, McMaster K, George D, Van Etten JL, Mead DA.

Gene. 1995 May 19;157(1-2):37-41.

PMID:
7607522
[PubMed - indexed for MEDLINE]
19.

SacNI, an isoschizomer of BanII isolated from Streptomyces achromogenes recognizes the 5'-GRGCY/C sequence.

Rutkowska SM, Skowron PM, Bielawski K, Podhajska AJ.

Gene. 1995 May 19;157(1-2):319-20.

PMID:
7607518
[PubMed - indexed for MEDLINE]
20.

Purification and characterization of the restriction endonuclease AvcI from Actinomyces cristalomycini.

Rutkowska SM, Skowron PM, Podhajska AJ.

Gene. 1995 May 19;157(1-2):317-8.

PMID:
7607517
[PubMed - indexed for MEDLINE]

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