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Results: 1 to 20 of 52

1.

Comparative Multi-Donor Study of IFNγ Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays.

Hagen J, Zimmerman R, Goetz C, Bonnevier J, Houchins JP, Reagan K, Kalyuzhny AE.

Cells. 2015 Feb 11;4(1):84-95. doi: 10.3390/cells4010084.

2.

A liquid chromatography-mass spectrometry-based approach to characterize the substrate specificity of mammalian heparanase.

Mao Y, Huang Y, Buczek-Thomas JA, Ethen CM, Nugent MA, Wu ZL, Zaia J.

J Biol Chem. 2014 Dec 5;289(49):34141-51. doi: 10.1074/jbc.M114.589630. Epub 2014 Oct 21.

PMID:
25336655
3.

Nonradioactive glycosyltransferase and sulfotransferase assay to study glycosaminoglycan biosynthesis.

Ethen CM, Machacek M, Prather B, Tatge T, Yu H, Wu ZL.

Methods Mol Biol. 2015;1229:431-41. doi: 10.1007/978-1-4939-1714-3_33.

PMID:
25325970
4.

Detecting O-GlcNAc using in vitro sulfation.

Wu ZL, Robey MT, Tatge T, Lin C, Leymarie N, Zou Y, Zaia J.

Glycobiology. 2014 Aug;24(8):740-7. doi: 10.1093/glycob/cwu037. Epub 2014 May 5.

PMID:
24799377
5.

Inhibition of SULT4A1 expression induces up-regulation of phototransduction gene expression in 72-hour postfertilization zebrafish larvae.

Crittenden F, Thomas H, Ethen CM, Wu ZL, Chen D, Kraft TW, Parant JM, Falany CN.

Drug Metab Dispos. 2014 May;42(5):947-53. doi: 10.1124/dmd.114.057042. Epub 2014 Feb 19.

6.

Induction and analysis of epithelial to mesenchymal transition.

Tang Y, Herr G, Johnson W, Resnik E, Aho J.

J Vis Exp. 2013 Aug 27;(78). doi: 10.3791/50478.

PMID:
24022340
7.

Golgi-resident PAP-specific 3'-phosphatase-coupled sulfotransferase assays.

Prather B, Ethen CM, Machacek M, Wu ZL.

Anal Biochem. 2012 Apr 1;423(1):86-92. doi: 10.1016/j.ab.2012.01.003. Epub 2012 Jan 17.

PMID:
22289690
8.

Combining ELISPOT and ELISA to measure amounts of cytokines secreted by a single cell.

Hagen J, Houchins JP, Kalyuzhny AE.

Methods Mol Biol. 2012;792:115-22. doi: 10.1007/978-1-61779-325-7_10.

PMID:
21956506
9.

ELISPOT assay for neuroscience research: studying TNFα secretion from microglial cells.

Hagen J, Houchins JP, Kalyuzhny AE.

Methods Mol Biol. 2012;792:97-104. doi: 10.1007/978-1-61779-325-7_8.

PMID:
21956504
10.

ELISPOT assay as a tool to study oxidative stress in lymphocytes.

Hagen J, Houchins JP, Kalyuzhny AE.

Methods Mol Biol. 2012;792:87-96. doi: 10.1007/978-1-61779-325-7_7.

PMID:
21956503
11.

Equine ELISPOT assay to study secretion of IFNγ and IL-4 from peripheral blood mononuclear cells.

Hagen J, Hartnett C, Houchins JP, Giguère S, Kalyuzhny AE.

Methods Mol Biol. 2012;792:39-45. doi: 10.1007/978-1-61779-325-7_3.

PMID:
21956499
12.

Sequential chromatin immunoprecipitation assay and analysis.

de Medeiros RB.

Methods Mol Biol. 2011;791:225-37. doi: 10.1007/978-1-61779-316-5_17.

PMID:
21913083
13.

Phosphatase-coupled universal kinase assay and kinetics for first-order-rate coupling reaction.

Wu ZL.

PLoS One. 2011;6(8):e23172. doi: 10.1371/journal.pone.0023172. Epub 2011 Aug 11.

14.

Absorption control in immunohistochemistry using phospho-peptides immobilized on magnetic beads.

Schoephoerster J, Frisch J, Grahek M, Wu C, He Y, Wang W, Nguyen J, Schwartz D, Kalyuzhny AE.

Methods Mol Biol. 2011;717:291-300. doi: 10.1007/978-1-61779-024-9_17.

PMID:
21370038
15.

Novel multicolor immunofluorescence technique using primary antibodies raised in the same host species.

Frisch J, Houchins JP, Grahek M, Schoephoerster J, Hagen J, Sweet J, Mendoza L, Schwartz D, Kalyuzhny AE.

Methods Mol Biol. 2011;717:233-44. doi: 10.1007/978-1-61779-024-9_13.

PMID:
21370034
16.

Overview of the generation, validation, and application of phosphosite-specific antibodies.

Brumbaugh K, Johnson W, Liao WC, Lin MS, Houchins JP, Cooper J, Stoesz S, Campos-Gonzalez R.

Methods Mol Biol. 2011;717:3-43. doi: 10.1007/978-1-61779-024-9_1. Review.

PMID:
21370022
17.

Detection of specific glycosaminoglycans and glycan epitopes by in vitro sulfation using recombinant sulfotransferases.

Wu ZL, Prather B, Ethen CM, Kalyuzhny A, Jiang W.

Glycobiology. 2011 May;21(5):625-33. doi: 10.1093/glycob/cwq204. Epub 2010 Dec 17.

18.

Universal phosphatase-coupled glycosyltransferase assay.

Wu ZL, Ethen CM, Prather B, Machacek M, Jiang W.

Glycobiology. 2011 Jun;21(6):727-33. doi: 10.1093/glycob/cwq187. Epub 2010 Nov 15.

19.

ELISpot assay as a tool to study oxidative stress in peripheral blood mononuclear cells.

Hagen J, Houchins JP, Kalyuzhny AE.

Methods Mol Biol. 2011;682:207-15. doi: 10.1007/978-1-60327-409-8_15.

PMID:
21057930
20.

Combination of TUNEL assay with immunohistochemistry for simultaneous detection of DNA fragmentation and oxidative cell damage.

Kalyuzhny AE.

Methods Mol Biol. 2011;682:15-27. doi: 10.1007/978-1-60327-409-8_2.

PMID:
21057917
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