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Items: 1 to 20 of 125

1.

Protein Stability in Reverse Micelles.

Senske M, Smith AE, Pielak GJ.

Angew Chem Int Ed Engl. 2016 Mar 7;55(11):3586-9. doi: 10.1002/anie.201508981. Epub 2016 Feb 8.

PMID:
26854977
2.

In-cell thermodynamics and a new role for protein surfaces.

Smith AE, Zhou LZ, Gorensek AH, Senske M, Pielak GJ.

Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):1725-30. doi: 10.1073/pnas.1518620113. Epub 2016 Jan 11.

PMID:
26755596
3.

Challenge of mimicking the influences of the cellular environment on RNA structure by PEG-induced macromolecular crowding.

Tyrrell J, Weeks KM, Pielak GJ.

Biochemistry. 2015 Oct 27;54(42):6447-53. doi: 10.1021/acs.biochem.5b00767. Epub 2015 Oct 15.

PMID:
26430778
4.

Intracellular pH modulates quinary structure.

Cohen RD, Guseman AJ, Pielak GJ.

Protein Sci. 2015 Nov;24(11):1748-55. doi: 10.1002/pro.2765. Epub 2015 Aug 30.

PMID:
26257390
5.

Emergence of life: Physical chemistry changes the paradigm.

Spitzer J, Pielak GJ, Poolman B.

Biol Direct. 2015 Jun 10;10:33. doi: 10.1186/s13062-015-0060-y. Review.

6.

Quinary structure modulates protein stability in cells.

Monteith WB, Cohen RD, Smith AE, Guzman-Cisneros E, Pielak GJ.

Proc Natl Acad Sci U S A. 2015 Feb 10;112(6):1739-42. doi: 10.1073/pnas.1417415112. Epub 2015 Jan 26.

7.

Hydrogen exchange of disordered proteins in Escherichia coli.

Smith AE, Zhou LZ, Pielak GJ.

Protein Sci. 2015 May;24(5):706-13. doi: 10.1002/pro.2643. Epub 2015 Mar 2.

8.

NMR studies of protein folding and binding in cells and cell-like environments.

Smith AE, Zhang Z, Pielak GJ, Li C.

Curr Opin Struct Biol. 2015 Feb;30:7-16. doi: 10.1016/j.sbi.2014.10.004. Epub 2014 Dec 3. Review.

PMID:
25479354
9.

Residue level quantification of protein stability in living cells.

Monteith WB, Pielak GJ.

Proc Natl Acad Sci U S A. 2014 Aug 5;111(31):11335-40. doi: 10.1073/pnas.1406845111. Epub 2014 Jul 21. Erratum in: Proc Natl Acad Sci U S A. 2015 Dec 15;112(50):E7031.

10.

An osmolyte mitigates the destabilizing effect of protein crowding.

Sarkar M, Pielak GJ.

Protein Sci. 2014 Sep;23(9):1161-4. doi: 10.1002/pro.2510. Epub 2014 Jul 15.

11.

Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs).

Theillet FX, Binolfi A, Frembgen-Kesner T, Hingorani K, Sarkar M, Kyne C, Li C, Crowley PB, Gierasch L, Pielak GJ, Elcock AH, Gershenson A, Selenko P.

Chem Rev. 2014 Jul 9;114(13):6661-714. doi: 10.1021/cr400695p. Epub 2014 Jun 5. Review. No abstract available.

12.

Strategies for protein NMR in Escherichia coli.

Xu G, Ye Y, Liu X, Cao S, Wu Q, Cheng K, Liu M, Pielak GJ, Li C.

Biochemistry. 2014 Apr 1;53(12):1971-81. doi: 10.1021/bi500079u. Epub 2014 Mar 20.

PMID:
24597855
13.

Protein crowder charge and protein stability.

Sarkar M, Lu J, Pielak GJ.

Biochemistry. 2014 Mar 18;53(10):1601-6. doi: 10.1021/bi4016346. Epub 2014 Mar 3.

PMID:
24552162
14.

Impact of reconstituted cytosol on protein stability.

Sarkar M, Smith AE, Pielak GJ.

Proc Natl Acad Sci U S A. 2013 Nov 26;110(48):19342-7. doi: 10.1073/pnas.1312678110. Epub 2013 Nov 11.

15.

The cellular environment stabilizes adenine riboswitch RNA structure.

Tyrrell J, McGinnis JL, Weeks KM, Pielak GJ.

Biochemistry. 2013 Dec 3;52(48):8777-85. doi: 10.1021/bi401207q. Epub 2013 Nov 20.

16.

(19) F NMR spectroscopy as a probe of cytoplasmic viscosity and weak protein interactions in living cells.

Ye Y, Liu X, Zhang Z, Wu Q, Jiang B, Jiang L, Zhang X, Liu M, Pielak GJ, Li C.

Chemistry. 2013 Sep 16;19(38):12705-10. doi: 10.1002/chem.201301657. Epub 2013 Aug 6.

PMID:
23922149
17.

Amide proton exchange of a dynamic loop in cell extracts.

Smith AE, Sarkar M, Young GB, Pielak GJ.

Protein Sci. 2013 Oct;22(10):1313-9. doi: 10.1002/pro.2318. Epub 2013 Aug 20.

18.

Disordered Protein Diffusion under Crowded Conditions.

Wang Y, Benton LA, Singh V, Pielak GJ.

J Phys Chem Lett. 2012 Sep 20;3(18):2703-2706. Epub 2012 Sep 6.

19.

Unexpected effects of macromolecular crowding on protein stability.

Benton LA, Smith AE, Young GB, Pielak GJ.

Biochemistry. 2012 Dec 11;51(49):9773-5. doi: 10.1021/bi300909q. Epub 2012 Nov 27.

PMID:
23167542
20.

Macromolecular crowding and protein stability.

Wang Y, Sarkar M, Smith AE, Krois AS, Pielak GJ.

J Am Chem Soc. 2012 Oct 10;134(40):16614-8. doi: 10.1021/ja305300m. Epub 2012 Sep 27.

PMID:
22954326
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