Progress in the development of Lactococcus lactis as a recombinant mucosal vaccine delivery system

Folia Microbiol (Praha). 1995;40(3):225-30. doi: 10.1007/BF02814197.

Abstract

The non-pathogenic, non-colonising Gram-positive organism Lactobacillus lactis is beeing developed as an antigen delivery system for mucosal vaccination. A high level expression system has been developed which allows loading of the bacterium with high levels of a heterologous antigen (TTFC) prior to inoculation. Mucosal inoculation of one such recombinant strain results in a protective serum antibody response and production of TTFC-specific IgA at mucosal sites.

Publication types

  • Review

MeSH terms

  • Animals
  • Antibodies, Bacterial / biosynthesis
  • Antigens, Bacterial / administration & dosage
  • Antigens, Bacterial / genetics
  • Genetic Vectors
  • Lactococcus lactis / genetics*
  • Lactococcus lactis / immunology*
  • Mucous Membrane / immunology
  • Peptide Fragments / administration & dosage
  • Peptide Fragments / genetics
  • Peptide Fragments / immunology
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Tetanus Toxin / administration & dosage
  • Tetanus Toxin / genetics
  • Tetanus Toxin / immunology
  • Vaccines, Synthetic / administration & dosage*
  • Vaccines, Synthetic / genetics

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Peptide Fragments
  • Recombinant Proteins
  • Tetanus Toxin
  • Vaccines, Synthetic
  • tetanus toxin fragment C