Abstract
The non-pathogenic, non-colonising Gram-positive organism Lactobacillus lactis is beeing developed as an antigen delivery system for mucosal vaccination. A high level expression system has been developed which allows loading of the bacterium with high levels of a heterologous antigen (TTFC) prior to inoculation. Mucosal inoculation of one such recombinant strain results in a protective serum antibody response and production of TTFC-specific IgA at mucosal sites.
MeSH terms
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Animals
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Antibodies, Bacterial / biosynthesis
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Antigens, Bacterial / administration & dosage
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Antigens, Bacterial / genetics
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Genetic Vectors
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Lactococcus lactis / genetics*
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Lactococcus lactis / immunology*
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Mucous Membrane / immunology
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Peptide Fragments / administration & dosage
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Peptide Fragments / genetics
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Peptide Fragments / immunology
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Recombinant Proteins / administration & dosage
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Tetanus Toxin / administration & dosage
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Tetanus Toxin / genetics
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Tetanus Toxin / immunology
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Vaccines, Synthetic / administration & dosage*
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Vaccines, Synthetic / genetics
Substances
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Antibodies, Bacterial
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Antigens, Bacterial
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Peptide Fragments
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Recombinant Proteins
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Tetanus Toxin
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Vaccines, Synthetic
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tetanus toxin fragment C