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Results: 1 to 20 of 124

1.

Detection of RNAs by 3'-End Labeling and RNase H Digestion.

Nilsen TW.

Cold Spring Harb Protoc. 2014 Jul 1;2014(7):pdb.prot080796. doi: 10.1101/pdb.prot080796.

PMID:
24987142
[PubMed - in process]
2.

Reverse footprinting to map sites of RNA-protein interactions.

Nilsen TW.

Cold Spring Harb Protoc. 2014 Jun 2;2014(6):683-6. doi: 10.1101/pdb.prot080804.

PMID:
24890211
[PubMed - in process]
3.

RNase footprinting to map sites of RNA-protein interactions.

Nilsen TW.

Cold Spring Harb Protoc. 2014 Jun 2;2014(6):677-82. doi: 10.1101/pdb.prot080788.

PMID:
24890210
[PubMed - in process]
4.

3'-end labeling of RNA with [5'-32P]Cytidine 3',5'-bis(phosphate) and T4 RNA ligase 1.

Nilsen TW.

Cold Spring Harb Protoc. 2014 Apr 1;2014(4):444-6. doi: 10.1101/pdb.prot080713.

PMID:
24692494
[PubMed - in process]
5.

Molecular biology. Internal mRNA methylation finally finds functions.

Nilsen TW.

Science. 2014 Mar 14;343(6176):1207-8. doi: 10.1126/science.1249340. No abstract available.

PMID:
24626918
[PubMed - indexed for MEDLINE]
6.

Preparing size markers for gel electrophoresis.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Dec 1;2013(12):1186-9. doi: 10.1101/pdb.prot079426.

PMID:
24298031
[PubMed - indexed for MEDLINE]
7.

RNA sequencing by primer extension.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Dec 1;2013(12):1182-5. doi: 10.1101/pdb.prot079400.

PMID:
24298029
[PubMed - indexed for MEDLINE]
8.

High-yield synthesis of RNA using T7 RNA polymerase and plasmid DNA or oligonucleotide templates.

Nilsen TW, Rio DC, Ares M Jr.

Cold Spring Harb Protoc. 2013 Nov 1;2013(11). pii: pdb.prot078535. doi: 10.1101/pdb.prot078535.

PMID:
24184762
[PubMed - indexed for MEDLINE]
9.

Toeprinting.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Sep 1;2013(9):896-9. doi: 10.1101/pdb.prot077180.

PMID:
24003204
[PubMed - indexed for MEDLINE]
10.

The fundamentals of RNA purification.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Jul 1;2013(7):618-24. doi: 10.1101/pdb.top075838.

PMID:
23818674
[PubMed - indexed for MEDLINE]
11.

Enzyme engineering through evolution: thermostable recombinant group II intron reverse transcriptases provide new tools for RNA research and biotechnology.

Collins K, Nilsen TW.

RNA. 2013 Aug;19(8):1017-8. doi: 10.1261/rna.040451.113. Epub 2013 Jun 21.

PMID:
23793892
[PubMed - indexed for MEDLINE]
Free Article
12.

Analysis of pre-mRNA splicing using HeLa cell nuclear extracts.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Jun 1;2013(6):584-7. doi: 10.1101/pdb.prot075184.

PMID:
23734029
[PubMed - indexed for MEDLINE]
13.

Preparation of Nuclear Extracts from HeLa cells.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Jun 1;2013(6):579-83. doi: 10.1101/pdb.prot075176.

PMID:
23734028
[PubMed - indexed for MEDLINE]
14.

RNA structure determination using nuclease digestion.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Apr 1;2013(4):379-82. doi: 10.1101/pdb.prot072330.

PMID:
23547152
[PubMed - indexed for MEDLINE]
15.

Detecting RNA-protein interactions by photocross-linking using RNA molecules containing uridine analogs.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Mar 1;2013(3). pii: pdb.prot073569. doi: 10.1101/pdb.prot073569.

PMID:
23457347
[PubMed - indexed for MEDLINE]
16.

Site-specific labeling of RNA.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Mar 1;2013(3). pii: pdb.prot072918. doi: 10.1101/pdb.prot072918.

PMID:
23457342
[PubMed - indexed for MEDLINE]
17.

Gel purification of RNA.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Feb 1;2013(2):180-3. doi: 10.1101/pdb.prot072942.

PMID:
23378657
[PubMed - indexed for MEDLINE]
18.

RNA-friendly plasmid preparation.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Feb 1;2013(2):174-9. doi: 10.1101/pdb.prot072926.

PMID:
23378655
[PubMed - indexed for MEDLINE]
19.

Determining the yield of RNA synthesized in vitro.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Jan 1;2013(1). pii: pdb.prot072629. doi: 10.1101/pdb.prot072629.

PMID:
23282637
[PubMed - indexed for MEDLINE]
20.

Splinted ligation method to detect small RNAs.

Nilsen TW.

Cold Spring Harb Protoc. 2013 Jan 1;2013(1). pii: pdb.prot072611. doi: 10.1101/pdb.prot072611.

PMID:
23282636
[PubMed - indexed for MEDLINE]

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