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Targeted gene editing by transfection of in vitro reconstituted Streptococcus thermophilus Cas9 nuclease complex.

Glemzaite M, Balciunaite E, Karvelis T, Gasiunas G, Grusyte MM, Alzbutas G, Jurcyte A, Anderson EM, Maksimova E, Smith AJ, Lubys A, Zaliauskiene L, Siksnys V.

RNA Biol. 2015;12(1):1-4. doi: 10.1080/15476286.2015.1017209. No abstract available.


Draft Genome Sequence of the Cyanobacterium Aphanizomenon flos-aquae Strain 2012/KM1/D3, Isolated from the Curonian Lagoon (Baltic Sea).

Šulčius S, Alzbutas G, Kvederavičiūtė K, Koreivienė J, Zakrys L, Lubys A, Paškauskas R.

Genome Announc. 2015 Jan 15;3(1). pii: e01392-14. doi: 10.1128/genomeA.01392-14.


A new prototype IIS/IIC/IIG endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus, recognising 5'-TARCCA(N11/9)-3' sequences.

Jezewska-Frackowiak J, Lubys A, Vitkute J, Zakareviciene L, Zebrowska J, Krefft D, Skowron MA, Zylicz-Stachula A, Skowron PM.

J Biotechnol. 2015 Jan 20;194:19-26. doi: 10.1016/j.jbiotec.2014.11.023. Epub 2014 Dec 4.


Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus.

Skowron PM, Vitkute J, Ramanauskaite D, Mitkaite G, Jezewska-Frackowiak J, Zebrowska J, Zylicz-Stachula A, Lubys A.

BMC Mol Biol. 2013 Aug 6;14:17. doi: 10.1186/1471-2199-14-17.


Screening for catalytically active Type II restriction endonucleases using segregation-induced methylation deficiency.

Ukanis M, Sapranauskas R, Lubys A.

Nucleic Acids Res. 2012 Oct;40(19):e149. doi: 10.1093/nar/gks635. Epub 2012 Jun 29.


Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities.

Zylicz-Stachula A, Zolnierkiewicz O, Lubys A, Ramanauskaite D, Mitkaite G, Bujnicki JM, Skowron PM.

BMC Mol Biol. 2012 Apr 10;13:13. doi: 10.1186/1471-2199-13-13.


Fluoride-cleavable, fluorescently labelled reversible terminators: synthesis and use in primer extension.

Knapp DC, Serva S, D'Onofrio J, Keller A, Lubys A, Kurg A, Remm M, Engels JW.

Chemistry. 2011 Mar 1;17(10):2903-15. doi: 10.1002/chem.201001952. Epub 2011 Feb 3.


Bioinformatic and partial functional analysis of pEspA and pEspB, two plasmids from Exiguobacterium arabatum sp. nov. RFL1109.

Jakubauskas A, Kriukiene E, Trinkunaite L, Sapranauskas R, Jurenaite-Urbanaviciene S, Lubys A.

Plasmid. 2009 Jan;61(1):52-64. doi: 10.1016/j.plasmid.2008.09.004. Epub 2008 Oct 26.


Synthesis of four colors fluorescently labelled 3'-O-blocked nucleotides with fluoride cleavable blocking group and linker for array based Sequencing-by-Synthesis applications.

Knapp DC, Keller A, D'Onofrio J, Lubys A, Serva S, Kurg A, Remm M, Kwiatkowski M, Engels JW.

Nucleic Acids Symp Ser (Oxf). 2008;(52):345-6. doi: 10.1093/nass/nrn174.


Generation of DNA cleavage specificities of type II restriction endonucleases by reassortment of target recognition domains.

Jurenaite-Urbanaviciene S, Serksnaite J, Kriukiene E, Giedriene J, Venclovas C, Lubys A.

Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10358-63. Epub 2007 Jun 6.


Identification of a new subfamily of HNH nucleases and experimental characterization of a representative member, HphI restriction endonuclease.

Cymerman IA, Obarska A, Skowronek KJ, Lubys A, Bujnicki JM.

Proteins. 2006 Dec 1;65(4):867-76.


Mva1269I: a monomeric type IIS restriction endonuclease from Micrococcus varians with two EcoRI- and FokI-like catalytic domains.

Armalyte E, Bujnicki JM, Giedriene J, Gasiunas G, Kosiński J, Lubys A.

J Biol Chem. 2005 Dec 16;280(50):41584-94. Epub 2005 Oct 11.


Random gene dissection: a tool for the investigation of protein structural organization.

Sapranauskas R, Lubys A.

Biotechniques. 2005 Sep;39(3):395-402.


MnlI--The member of H-N-H subtype of Type IIS restriction endonucleases.

Kriukiene E, Lubiene J, Lagunavicius A, Lubys A.

Biochim Biophys Acta. 2005 Aug 10;1751(2):194-204.


Esp1396I restriction-modification system: structural organization and mode of regulation.

Cesnaviciene E, Mitkaite G, Stankevicius K, Janulaitis A, Lubys A.

Nucleic Acids Res. 2003 Jan 15;31(2):743-9.


Evolutionary relationship of Alw26I, Eco31I and Esp3I, restriction endonucleases that recognise overlapping sequences.

Bitinaite J, Mitkaite G, Dauksaite V, Jakubauskas A, Timinskas A, Vaisvila R, Lubys A, Janulaitis A.

Mol Genet Genomics. 2002 Jul;267(5):664-72. Epub 2002 Jun 19.


Circular permutation of DNA cytosine-N4 methyltransferases: in vivo coexistence in the BcnI system and in vitro probing by hybrid formation.

Vilkaitis G, Lubys A, Merkiene E, Timinskas A, Janulaitis A, Klimasauskas S.

Nucleic Acids Res. 2002 Apr 1;30(7):1547-57.


Characterization of AloI, a restriction-modification system of a new type.

Cesnaviciene E, Petrusyte M, Kazlauskiene R, Maneliene Z, Timinskas A, Lubys A, Janulaitis A.

J Mol Biol. 2001 Nov 23;314(2):205-16.


Characterization of BseMII, a new type IV restriction-modification system, which recognizes the pentanucleotide sequence 5'-CTCAG(N)(10/8)/.

Jurenaite-Urbanaviciene S, Kazlauskiene R, Urbelyte V, Maneliene Z, Petrusyte M, Lubys A, Janulaitis A.

Nucleic Acids Res. 2001 Feb 15;29(4):895-903.

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