Abstract
Biological activity of protein extracts from transgenic plants of chicory Cichorium intybus L. and lettuce Lactuca sativa L. with human interferon alpha2b gene was investigated against vesicular stomatitis virus. It was shown that the extracts from the hairy roots of chicory and lettuce transformed by A. rhizogenes possess the antiviral activity 1620...5400 IU/g weight, and the extracts from leaves of the plants transformed by A. tumefaciens--till 9375 IU/g weight. Dependence of plant extract biological activity on the transformation vector was shown.
MeSH terms
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Agrobacterium / genetics
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Agrobacterium tumefaciens / genetics
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Animals
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Antiviral Agents / isolation & purification
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Antiviral Agents / pharmacology*
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Cattle
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Cell Line
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Cichorium intybus / genetics*
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Genetic Engineering
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Genetic Vectors
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Humans
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Interferon alpha-2
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Interferon-alpha / isolation & purification
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Interferon-alpha / pharmacology*
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Lactuca / genetics*
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Plant Leaves / chemistry
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Plant Leaves / genetics
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Plant Roots / chemistry
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Plant Roots / genetics*
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Plants, Genetically Modified
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Plasmids
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / pharmacology
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Reverse Transcriptase Polymerase Chain Reaction
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Species Specificity
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Vesiculovirus / drug effects*
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Vesiculovirus / growth & development
Substances
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Antiviral Agents
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Interferon alpha-2
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Interferon-alpha
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Recombinant Proteins