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Results: 1 to 20 of 61

1.

Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples.

Sanders R, Mason DJ, Foy CA, Huggett JF.

Anal Bioanal Chem. 2014 May 25. [Epub ahead of print]

PMID:
24858468
[PubMed - as supplied by publisher]
2.

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification.

Devonshire AS, Whale AS, Gutteridge A, Jones G, Cowen S, Foy CA, Huggett JF.

Anal Bioanal Chem. 2014 May 24. [Epub ahead of print]

PMID:
24853859
[PubMed - as supplied by publisher]
3.

Minimum information necessary for quantitative real-time PCR experiments.

Johnson G, Nour AA, Nolan T, Huggett J, Bustin S.

Methods Mol Biol. 2014;1160:5-17. doi: 10.1007/978-1-4939-0733-5_2.

PMID:
24740217
[PubMed - in process]
4.

Comparative study of sensitivity, linearity, and resistance to inhibition of digital and nondigital polymerase chain reaction and loop mediated isothermal amplification assays for quantification of human cytomegalovirus.

Nixon G, Garson JA, Grant P, Nastouli E, Foy CA, Huggett JF.

Anal Chem. 2014 May 6;86(9):4387-94. doi: 10.1021/ac500208w. Epub 2014 Apr 22.

PMID:
24684191
[PubMed - in process]
5.

The need for transparency and good practices in the qPCR literature.

Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT, Schjerling P, Day PJ, Abreu M, Aguado B, Beaulieu JF, Beckers A, Bogaert S, Browne JA, Carrasco-Ramiro F, Ceelen L, Ciborowski K, Cornillie P, Coulon S, Cuypers A, De Brouwer S, De Ceuninck L, De Craene J, De Naeyer H, De Spiegelaere W, Deckers K, Dheedene A, Durinck K, Ferreira-Teixeira M, Fieuw A, Gallup JM, Gonzalo-Flores S, Goossens K, Heindryckx F, Herring E, Hoenicka H, Icardi L, Jaggi R, Javad F, Karampelias M, Kibenge F, Kibenge M, Kumps C, Lambertz I, Lammens T, Markey A, Messiaen P, Mets E, Morais S, Mudarra-Rubio A, Nakiwala J, Nelis H, Olsvik PA, Pérez-Novo C, Plusquin M, Remans T, Rihani A, Rodrigues-Santos P, Rondou P, Sanders R, Schmidt-Bleek K, Skovgaard K, Smeets K, Tabera L, Toegel S, Van Acker T, Van den Broeck W, Van der Meulen J, Van Gele M, Van Peer G, Van Poucke M, Van Roy N, Vergult S, Wauman J, Tshuikina-Wiklander M, Willems E, Zaccara S, Zeka F, Vandesompele J.

Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697.

PMID:
24173381
[PubMed - indexed for MEDLINE]
6.

Digital PCR as a novel technology and its potential implications for molecular diagnostics.

Huggett JF, Whale A.

Clin Chem. 2013 Dec;59(12):1691-3. doi: 10.1373/clinchem.2013.214742. Epub 2013 Oct 7. No abstract available.

PMID:
24100808
[PubMed - indexed for MEDLINE]
7.

Evaluation of digital PCR for absolute RNA quantification.

Sanders R, Mason DJ, Foy CA, Huggett JF.

PLoS One. 2013 Sep 20;8(9):e75296. doi: 10.1371/journal.pone.0075296. eCollection 2013.

PMID:
24073259
[PubMed - indexed for MEDLINE]
Free PMC Article
8.

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA.

Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9.

PMID:
23570709
[PubMed - indexed for MEDLINE]
Free Article
9.

Phage display of functional αβ single-chain T-cell receptor molecules specific for CD1b:Ac₂SGL complexes from Mycobacterium tuberculosis-infected cells.

Camacho F, Huggett J, Kim L, Infante JF, Lepore M, Perez V, Sarmiento ME, Rook G, Acosta A.

BMC Immunol. 2013;14 Suppl 1:S2. doi: 10.1186/1471-2172-14-S1-S2. Epub 2013 Feb 25.

PMID:
23458512
[PubMed - indexed for MEDLINE]
Free PMC Article
10.

A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability.

Redshaw N, Wilkes T, Whale A, Cowen S, Huggett J, Foy CA.

Biotechniques. 2013 Mar;54(3):155-64. doi: 10.2144/000114002.

PMID:
23477383
[PubMed - indexed for MEDLINE]
Free Article
11.

Methods for applying accurate digital PCR analysis on low copy DNA samples.

Whale AS, Cowen S, Foy CA, Huggett JF.

PLoS One. 2013;8(3):e58177. doi: 10.1371/journal.pone.0058177. Epub 2013 Mar 5.

PMID:
23472156
[PubMed - indexed for MEDLINE]
Free PMC Article
12.

Progress in metagenomics requires a balanced appraisal of the available technologies.

Huggett JF, Studholme DJ, Laver T, Foy CA.

Eur J Clin Microbiol Infect Dis. 2013 Aug;32(8):1097-8. doi: 10.1007/s10096-013-1842-8. Epub 2013 Feb 23. No abstract available.

PMID:
23435754
[PubMed - indexed for MEDLINE]
13.

A phantom-based evaluation of three commercially available patient organ shields for computed tomography X-ray examinations in diagnostic radiology.

Huggett J, Mukonoweshuro W, Loader R.

Radiat Prot Dosimetry. 2013 Jul;155(2):161-8. doi: 10.1093/rpd/ncs327. Epub 2012 Dec 5.

PMID:
23222552
[PubMed - indexed for MEDLINE]
14.

Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis.

Devonshire AS, Sanders R, Wilkes TM, Taylor MS, Foy CA, Huggett JF.

Methods. 2013 Jan;59(1):89-100. doi: 10.1016/j.ymeth.2012.07.021. Epub 2012 Jul 24. Review.

PMID:
22841564
[PubMed - indexed for MEDLINE]
15.

Quality assessment of biobanked nucleic acid extracts for downstream molecular analysis.

Wahlberg K, Huggett J, Sanders R, Whale AS, Bushell C, Elaswarapu R, Scott DJ, Foy CA.

Biopreserv Biobank. 2012 Jun;10(3):266-75. doi: 10.1089/bio.2012.0004.

PMID:
24835065
[PubMed - in process]
16.

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation.

Whale AS, Huggett JF, Cowen S, Speirs V, Shaw J, Ellison S, Foy CA, Scott DJ.

Nucleic Acids Res. 2012 Jun;40(11):e82. doi: 10.1093/nar/gks203. Epub 2012 Feb 28.

PMID:
22373922
[PubMed - indexed for MEDLINE]
Free PMC Article
17.

Rapid and accurate detection of Mycobacterium tuberculosis in sputum samples by Cepheid Xpert MTB/RIF assay--a clinical validation study.

Rachow A, Zumla A, Heinrich N, Rojas-Ponce G, Mtafya B, Reither K, Ntinginya EN, O'Grady J, Huggett J, Dheda K, Boehme C, Perkins M, Saathoff E, Hoelscher M.

PLoS One. 2011;6(6):e20458. doi: 10.1371/journal.pone.0020458. Epub 2011 Jun 29.

PMID:
21738575
[PubMed - indexed for MEDLINE]
Free PMC Article
18.

Evaluation of digital PCR for absolute DNA quantification.

Sanders R, Huggett JF, Bushell CA, Cowen S, Scott DJ, Foy CA.

Anal Chem. 2011 Sep 1;83(17):6474-84. doi: 10.1021/ac103230c. Epub 2011 Aug 1.

PMID:
21446772
[PubMed - indexed for MEDLINE]
19.

Primer sequence disclosure: a clarification of the MIQE guidelines.

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT.

Clin Chem. 2011 Jun;57(6):919-21. doi: 10.1373/clinchem.2011.162958. Epub 2011 Mar 18. No abstract available.

PMID:
21421813
[PubMed - indexed for MEDLINE]
Free Article
20.

Accommodating dynamic oceanographic processes and pelagic biodiversity in marine conservation planning.

Grantham HS, Game ET, Lombard AT, Hobday AJ, Richardson AJ, Beckley LE, Pressey RL, Huggett JA, Coetzee JC, van der Lingen CD, Petersen SL, Merkle D, Possingham HP.

PLoS One. 2011 Feb 2;6(2):e16552. doi: 10.1371/journal.pone.0016552.

PMID:
21311757
[PubMed - indexed for MEDLINE]
Free PMC Article

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