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Results: 1 to 20 of 67

1.

Highly Reproducible Absolute Quantification of Mycobacterium tuberculosis Complex by Digital PCR.

Devonshire AS, Honeyborne I, Gutteridge A, Whale AS, Nixon G, Wilson P, Jones G, McHugh TD, Foy CA, Huggett JF.

Anal Chem. 2015 Mar 10. [Epub ahead of print]

PMID:
25646934
2.

Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis.

Redshaw N, Huggett JF, Taylor MS, Foy CA, Devonshire AS.

BMC Genomics. 2014 Dec 23;15(1):1174. [Epub ahead of print]

3.

Assessing the accuracy of quantitative molecular microbial profiling.

O'Sullivan DM, Laver T, Temisak S, Redshaw N, Harris KA, Foy CA, Studholme DJ, Huggett JF.

Int J Mol Sci. 2014 Nov 21;15(11):21476-91. doi: 10.3390/ijms151121476.

4.

STANDARDISING CLINICAL MEASUREMENTS OF BACTERIA AND VIRUSES USING NUCLEIC ACID TESTS.

Pavšič J, Devonshire AS, Parkes H, Schimmel H, Foy CA, Karczmarczyk M, Gutiérrez-Aguirre I, Honeyborne I, Huggett JF, McHugh TD, Milavec M, Zeichhardt H, Zel J.

J Clin Microbiol. 2014 Nov 12. pii: JCM.02136-14. [Epub ahead of print]

PMID:
25392365
5.

Considerations for digital PCR as an accurate molecular diagnostic tool.

Huggett JF, Cowen S, Foy CA.

Clin Chem. 2015 Jan;61(1):79-88. doi: 10.1373/clinchem.2014.221366. Epub 2014 Oct 22. Review.

PMID:
25338683
6.

Clinical features, microbiology and surgical outcomes of infective endocarditis: a 13-year study from a UK tertiary cardiothoracic referral centre.

Marks DJ, Hyams C, Koo CY, Pavlou M, Robbins J, Koo CS, Rodger G, Huggett JF, Yap J, Macrae MB, Swanton RH, Zumla AI, Miller RF.

QJM. 2015 Mar;108(3):219-29. doi: 10.1093/qjmed/hcu188. Epub 2014 Sep 15.

PMID:
25223570
7.

Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples.

Sanders R, Mason DJ, Foy CA, Huggett JF.

Anal Bioanal Chem. 2014 Oct;406(26):6471-83. doi: 10.1007/s00216-014-7857-x. Epub 2014 May 25.

8.

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification.

Devonshire AS, Whale AS, Gutteridge A, Jones G, Cowen S, Foy CA, Huggett JF.

Anal Bioanal Chem. 2014 Oct;406(26):6499-512. doi: 10.1007/s00216-014-7835-3. Epub 2014 May 24.

9.

Minimum information necessary for quantitative real-time PCR experiments.

Johnson G, Nour AA, Nolan T, Huggett J, Bustin S.

Methods Mol Biol. 2014;1160:5-17. doi: 10.1007/978-1-4939-0733-5_2.

PMID:
24740217
10.
11.

The need for transparency and good practices in the qPCR literature.

Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT, Schjerling P, Day PJ, Abreu M, Aguado B, Beaulieu JF, Beckers A, Bogaert S, Browne JA, Carrasco-Ramiro F, Ceelen L, Ciborowski K, Cornillie P, Coulon S, Cuypers A, De Brouwer S, De Ceuninck L, De Craene J, De Naeyer H, De Spiegelaere W, Deckers K, Dheedene A, Durinck K, Ferreira-Teixeira M, Fieuw A, Gallup JM, Gonzalo-Flores S, Goossens K, Heindryckx F, Herring E, Hoenicka H, Icardi L, Jaggi R, Javad F, Karampelias M, Kibenge F, Kibenge M, Kumps C, Lambertz I, Lammens T, Markey A, Messiaen P, Mets E, Morais S, Mudarra-Rubio A, Nakiwala J, Nelis H, Olsvik PA, Pérez-Novo C, Plusquin M, Remans T, Rihani A, Rodrigues-Santos P, Rondou P, Sanders R, Schmidt-Bleek K, Skovgaard K, Smeets K, Tabera L, Toegel S, Van Acker T, Van den Broeck W, Van der Meulen J, Van Gele M, Van Peer G, Van Poucke M, Van Roy N, Vergult S, Wauman J, Tshuikina-Wiklander M, Willems E, Zaccara S, Zeka F, Vandesompele J.

Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697.

PMID:
24173381
12.

Digital PCR as a novel technology and its potential implications for molecular diagnostics.

Huggett JF, Whale A.

Clin Chem. 2013 Dec;59(12):1691-3. doi: 10.1373/clinchem.2013.214742. Epub 2013 Oct 7. No abstract available.

13.

Evaluation of digital PCR for absolute RNA quantification.

Sanders R, Mason DJ, Foy CA, Huggett JF.

PLoS One. 2013 Sep 20;8(9):e75296. doi: 10.1371/journal.pone.0075296. eCollection 2013.

14.

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA.

Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9.

15.

Phage display of functional αβ single-chain T-cell receptor molecules specific for CD1b:Ac₂SGL complexes from Mycobacterium tuberculosis-infected cells.

Camacho F, Huggett J, Kim L, Infante JF, Lepore M, Perez V, Sarmiento ME, Rook G, Acosta A.

BMC Immunol. 2013;14 Suppl 1:S2. doi: 10.1186/1471-2172-14-S1-S2. Epub 2013 Feb 25.

16.

A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability.

Redshaw N, Wilkes T, Whale A, Cowen S, Huggett J, Foy CA.

Biotechniques. 2013 Mar;54(3):155-64. doi: 10.2144/000114002.

17.

Methods for applying accurate digital PCR analysis on low copy DNA samples.

Whale AS, Cowen S, Foy CA, Huggett JF.

PLoS One. 2013;8(3):e58177. doi: 10.1371/journal.pone.0058177. Epub 2013 Mar 5.

18.

Progress in metagenomics requires a balanced appraisal of the available technologies.

Huggett JF, Studholme DJ, Laver T, Foy CA.

Eur J Clin Microbiol Infect Dis. 2013 Aug;32(8):1097-8. doi: 10.1007/s10096-013-1842-8. Epub 2013 Feb 23. No abstract available.

PMID:
23435754
19.

A phantom-based evaluation of three commercially available patient organ shields for computed tomography X-ray examinations in diagnostic radiology.

Huggett J, Mukonoweshuro W, Loader R.

Radiat Prot Dosimetry. 2013 Jul;155(2):161-8. doi: 10.1093/rpd/ncs327. Epub 2012 Dec 5.

PMID:
23222552
20.

Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis.

Devonshire AS, Sanders R, Wilkes TM, Taylor MS, Foy CA, Huggett JF.

Methods. 2013 Jan;59(1):89-100. doi: 10.1016/j.ymeth.2012.07.021. Epub 2012 Jul 24. Review.

PMID:
22841564
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