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A Rapid and Low-Cost PCR Thermal Cycler for Infectious Disease Diagnostics.

Chan K, Wong PY, Yu P, Hardick J, Wong KY, Wilson SA, Wu T, Hui Z, Gaydos C, Wong SS.

PLoS One. 2016 Feb 12;11(2):e0149150. doi: 10.1371/journal.pone.0149150. eCollection 2016.


Sequencing Ebola and Marburg viruses genomes using microarrays.

Hardick J, Woelfel R, Gardner W, Ibrahim S.

J Med Virol. 2016 Jan 29. doi: 10.1002/jmv.24487. [Epub ahead of print]


Nested Machine Learning Facilitates Increased Sequence Content for Large-Scale Automated High Resolution Melt Genotyping.

Fraley SI, Athamanolap P, Masek BJ, Hardick J, Carroll KC, Hsieh YH, Rothman RE, Gaydos CA, Wang TH, Yang S.

Sci Rep. 2016 Jan 18;6:19218. doi: 10.1038/srep19218.


Universal digital high-resolution melt: a novel approach to broad-based profiling of heterogeneous biological samples.

Fraley SI, Hardick J, Masek BJ, Athamanolap P, Rothman RE, Gaydos CA, Carroll KC, Wakefield T, Wang TH, Yang S.

Nucleic Acids Res. 2016 Jan 8;44(1):508. doi: 10.1093/nar/gkv1083. Epub 2015 Oct 17. No abstract available.


Prospective comparison of RT-PCR/ESI-MS to Prodesse ProFlu Plus and Cepheid GenXpert for the detection of Influenza A and B viruses.

Hardick J, Dugas A, Goheen J, Rothman R, Gaydos C.

J Virol Methods. 2015 Mar;214:43-5. doi: 10.1016/j.jviromet.2015.01.002. Epub 2015 Feb 11.


Evaluation of the Xpert Flu rapid PCR assay in high-risk emergency department patients.

Dugas AF, Valsamakis A, Gaydos CA, Forman M, Hardick J, Kidambi P, Amin S, Gupta A, Rothman RE.

J Clin Microbiol. 2014 Dec;52(12):4353-5. doi: 10.1128/JCM.02343-14. Epub 2014 Sep 24.


A rabbit model of non-typhoidal Salmonella bacteremia.

Panda A, Tatarov I, Masek BJ, Hardick J, Crusan A, Wakefield T, Carroll K, Yang S, Hsieh YH, Lipsky MM, McLeod CG, Levine MM, Rothman RE, Gaydos CA, DeTolla LJ.

Comp Immunol Microbiol Infect Dis. 2014 Sep;37(4):211-20. doi: 10.1016/j.cimid.2014.05.004. Epub 2014 Jul 3.


A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR.

Hardick J, Sadiq S, Perelstein E, Peterson S, Rothman R, Gaydos CA.

Diagn Microbiol Infect Dis. 2014 Jun;79(2):187-9. doi: 10.1016/j.diagmicrobio.2014.02.009. Epub 2014 Feb 24.


Point of care diagnostics for sexually transmitted infections: perspectives and advances.

Gaydos C, Hardick J.

Expert Rev Anti Infect Ther. 2014 Jun;12(6):657-72. doi: 10.1586/14787210.2014.880651. Epub 2014 Feb 3. Review.


Is there evidence for resistance of ocular Chlamydia trachomatis to azithromycin after mass treatment for trachoma control?

West SK, Moncada J, Munoz B, Mkocha H, Storey P, Hardick J, Gaydos CA, Quinn TC, Schachter J.

J Infect Dis. 2014 Jul 1;210(1):65-71. Epub 2014 Jan 19.


Sensitive detection and serovar differentiation of typhoidal and nontyphoidal Salmonella enterica species using 16S rRNA Gene PCR coupled with high-resolution melt analysis.

Masek BJ, Hardick J, Won H, Yang S, Hsieh YH, Rothman RE, Gaydos CA.

J Mol Diagn. 2014 Mar;16(2):261-6. doi: 10.1016/j.jmoldx.2013.10.011. Epub 2013 Dec 21.


Universal digital high-resolution melt: a novel approach to broad-based profiling of heterogeneous biological samples.

Fraley SI, Hardick J, Jo Masek B, Athamanolap P, Rothman RE, Gaydos CA, Carroll KC, Wakefield T, Wang TH, Yang S.

Nucleic Acids Res. 2013 Oct;41(18):e175. doi: 10.1093/nar/gkt684. Epub 2013 Aug 9. Erratum in: Nucleic Acids Res. 2016 Jan 8;44(1):508.


Reverse transcription-PCR-electrospray ionization mass spectrometry for rapid detection of biothreat and common respiratory pathogens.

Jeng K, Hardick J, Rothman R, Yang S, Won H, Peterson S, Hsieh YH, Masek BJ, Carroll KC, Gaydos CA.

J Clin Microbiol. 2013 Oct;51(10):3300-7. doi: 10.1128/JCM.01443-13. Epub 2013 Jul 31.


Evaluation of the Pan Influenza detection kit utilizing the PLEX-ID and influenza samples from the 2011 respiratory season.

A Murillo L, Hardick J, Jeng K, Gaydos CA.

J Virol Methods. 2013 Oct;193(1):173-6. doi: 10.1016/j.jviromet.2013.06.006. Epub 2013 Jun 10.


A new rapid molecular point-of-care assay for Trichomonas vaginalis: preliminary performance data.

Pearce DM, Styles DN, Hardick JP, Gaydos CA.

Sex Transm Infect. 2013 Sep;89(6):495-7. doi: 10.1136/sextrans-2012-051000. Epub 2013 Apr 20.


Harnessing genomic approaches for infectious disease diagnosis in emergency medicine: getting closer to prime time.

Rothman RE, Yang S, Hardick J, Gaydos CA.

Ann Emerg Med. 2012 Nov;60(5):621-3. doi: 10.1016/j.annemergmed.2012.07.109. Epub 2012 Aug 22. No abstract available.


Chlamydia trachomatis strains show specific clustering for men who have sex with men compared to heterosexual populations in Sweden, the Netherlands, and the United States.

Christerson L, Bom RJ, Bruisten SM, Yass R, Hardick J, Bratt G, Gaydos CA, Morré SA, Herrmann B.

J Clin Microbiol. 2012 Nov;50(11):3548-55. doi: 10.1128/JCM.01713-12. Epub 2012 Aug 22.


Comparative analysis of two broad-range PCR assays for pathogen detection in positive-blood-culture bottles: PCR-high-resolution melting analysis versus PCR-mass spectrometry.

Jeng K, Gaydos CA, Blyn LB, Yang S, Won H, Matthews H, Toleno D, Hsieh YH, Carroll KC, Hardick J, Masek B, Kecojevic A, Sampath R, Peterson S, Rothman RE.

J Clin Microbiol. 2012 Oct;50(10):3287-92. doi: 10.1128/JCM.00677-12. Epub 2012 Aug 1.


A broad range assay for rapid detection and etiologic characterization of bacterial meningitis: performance testing in samples from sub-Sahara.

Won H, Yang S, Gaydos C, Hardick J, Ramachandran P, Hsieh YH, Kecojevic A, Njanpop-Lafourcade BM, Mueller JE, Tameklo TA, Badziklou K, Gessner BD, Rothman RE.

Diagn Microbiol Infect Dis. 2012 Sep;74(1):22-7. doi: 10.1016/j.diagmicrobio.2012.05.015. Epub 2012 Jul 17.


Monitoring seasonal influenza A evolution: rapid 2009 pandemic H1N1 surveillance with an reverse transcription-polymerase chain reaction/electro-spray ionization mass spectrometry assay.

Jeng K, Massire C, Zembower TR, Deyde VM, Gubareva LV, Hsieh YH, Rothman RE, Sampath R, Penugonda S, Metzgar D, Blyn LB, Hardick J, Gaydos CA.

J Clin Virol. 2012 Aug;54(4):332-6. doi: 10.1016/j.jcv.2012.05.002. Epub 2012 Jun 4.

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