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Results: 13


Expression, purification, and molecular analysis of the Necator americanus glutathione S-transferase 1 (Na-GST-1): a production process developed for a lead candidate recombinant hookworm vaccine antigen.

Goud GN, Deumic V, Gupta R, Brelsford J, Zhan B, Gillespie P, Plieskatt JL, Tsao EI, Hotez PJ, Bottazzi ME.

Protein Expr Purif. 2012 Jun;83(2):145-51. doi: 10.1016/j.pep.2012.03.013. Epub 2012 Apr 4.


Crystallization and preliminary X-ray analysis of Na-SAA-2 from the human hookworm parasite Necator americanus.

Asojo OA, Goud GN, Zhan B, Ordonez K, Sedlacek M, Homma K, Deumic V, Gupta R, Brelsford J, Price MK, Ngamelue MN, Hotez PJ.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt 2):172-6. doi: 10.1107/S1744309109051616. Epub 2010 Jan 27.


The evaluation of recombinant hookworm antigens as vaccines in hamsters (Mesocricetus auratus) challenged with human hookworm, Necator americanus.

Xiao S, Zhan B, Xue J, Goud GN, Loukas A, Liu Y, Williamson A, Liu S, Deumic V, Hotez P.

Exp Parasitol. 2008 Jan;118(1):32-40. Epub 2007 Jun 6.


X-ray structures of Na-GST-1 and Na-GST-2 two glutathione S-transferase from the human hookworm Necator americanus.

Asojo OA, Homma K, Sedlacek M, Ngamelue M, Goud GN, Zhan B, Deumic V, Asojo O, Hotez PJ.

BMC Struct Biol. 2007 Jun 26;7:42.


Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs.

Loukas A, Bethony JM, Mendez S, Fujiwara RT, Goud GN, Ranjit N, Zhan B, Jones K, Bottazzi ME, Hotez PJ.

PLoS Med. 2005 Oct;2(10):e295. Epub 2005 Oct 4.


Expression of the Necator americanus hookworm larval antigen Na-ASP-2 in Pichia pastoris and purification of the recombinant protein for use in human clinical trials.

Goud GN, Bottazzi ME, Zhan B, Mendez S, Deumic V, Plieskatt J, Liu S, Wang Y, Bueno L, Fujiwara R, Samuel A, Ahn SY, Solanki M, Asojo OA, Wang J, Bethony JM, Loukas A, Roy M, Hotez PJ.

Vaccine. 2005 Sep 15;23(39):4754-64.


Cloning and characterisation of an aspartyl protease inhibitor (API-1) from Ancylostoma hookworms.

Delaney A, Williamson A, Brand A, Ashcom J, Varghese G, Goud GN, Hawdon JM.

Int J Parasitol. 2005 Mar;35(3):303-13. Epub 2005 Jan 8.


Vaccination of dogs with a recombinant cysteine protease from the intestine of canine hookworms diminishes the fecundity and growth of worms.

Loukas A, Bethony JM, Williamson AL, Goud GN, Mendez S, Zhan B, Hawdon JM, Elena Bottazzi M, Brindley PJ, Hotez PJ.

J Infect Dis. 2004 May 15;189(10):1952-61. Epub 2004 Apr 27.


Cloning, yeast expression, isolation, and vaccine testing of recombinant Ancylostoma-secreted protein (ASP)-1 and ASP-2 from Ancylostoma ceylanicum.

Goud GN, Zhan B, Ghosh K, Loukas A, Hawdon J, Dobardzic A, Deumic V, Liu S, Dobardzic R, Zook BC, Jin Q, Liu Y, Hoffman L, Chung-Debose S, Patel R, Mendez S, Hotez PJ.

J Infect Dis. 2004 Mar 1;189(5):919-29. Epub 2004 Feb 6.


Progress in the development of a recombinant vaccine for human hookworm disease: the Human Hookworm Vaccine Initiative.

Hotez PJ, Zhan B, Bethony JM, Loukas A, Williamson A, Goud GN, Hawdon JM, Dobardzic A, Dobardzic R, Ghosh K, Bottazzi ME, Mendez S, Zook B, Wang Y, Liu S, Essiet-Gibson I, Chung-Debose S, Xiao S, Knox D, Meagher M, Inan M, Correa-Oliveira R, Vilk P, Shepherd HR, Brandt W, Russell PK.

Int J Parasitol. 2003 Sep 30;33(11):1245-58. Review.


Specific glycosidase activity isolated from a random phage display antibody library.

Goud GN, Artsaenko O, Bols M, Sierks M.

Biotechnol Prog. 2001 Jan-Feb;17(1):197-202.


Selection of optimum affinity tags from a phage-displayed peptide library. Application to immobilized copper(II) affinity chromatography.

Patwardhan AV, Goud GN, Koepsel RR, Ataai MM.

J Chromatogr A. 1997 Nov 7;787(1-2):91-100.

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