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Chemical dephosphorylation for identification of multiply phosphorylated peptides and phosphorylation site determination.
Kyono Y, Sugiyama N, Tomita M, Ishihama Y. Kyono Y, et al. Rapid Commun Mass Spectrom. 2010 Aug 15;24(15):2277-82. doi: 10.1002/rcm.4627. Rapid Commun Mass Spectrom. 2010. PMID: 20623713
We have developed a novel strategy to improve the efficiency of identification of multiply phosphorylated peptides isolated by hydroxy acid modified metal oxide chromatography (HAMMOC). ...These results indicate that chemical dephosphorylatio
We have developed a novel strategy to improve the efficiency of identification of multiply phosphorylated peptides
Confident site localization using a simulated phosphopeptide spectral library.
Suni V, Imanishi SY, Maiolica A, Aebersold R, Corthals GL. Suni V, et al. J Proteome Res. 2015 May 1;14(5):2348-59. doi: 10.1021/acs.jproteome.5b00050. Epub 2015 Mar 27. J Proteome Res. 2015. PMID: 25774671
We have investigated if phosphopeptide identification and simultaneous site localization can be achieved by spectral library searching. ...For future development, simulation of multiply phosphorylated peptides remains to be implemented....
We have investigated if phosphopeptide identification and simultaneous site localization can be achieved by spectral library s …