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Protein Eng Des Sel. 2011 Jun;24(6):525-30. doi: 10.1093/protein/gzr008. Epub 2011 Mar 14.

Secretion-and-capture cell-surface display for selection of target-binding proteins.

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  • 1Celexion LLC, One Kendall Square, Suite 14302, Cambridge, MA 02139, USA. arakestraw@celexionbio.com

Abstract

This report describes a new cell-surface display system, the Secretion and Capture Technology (SECANTâ„¢) platform, which relies on in vivo biotinylation of the protein of interest followed by its capture on the avidinated surface of the parent cell. Cell sorting techniques are then used to isolate clones that display target-binding protein. A distinguishing feature of this method is its ability to display complex proteins, such as full-length immunoglobulin G (IgG) antibodies, on living cells. In this proof-of-concept study, Saccharomyces cerevisiae cells that displayed Herceptin IgG were isolated from a 10,000-fold excess of cells that displayed a lysozyme-binding antibody.

PMID:
21402751
[PubMed - indexed for MEDLINE]
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