Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia

Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15524-9. doi: 10.1073/pnas.242606799. Epub 2002 Nov 14.

Abstract

Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors ( approximately 70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority ( approximately 68%) of CLL cases.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • B-Lymphocytes / chemistry
  • B-Lymphocytes / cytology
  • Blotting, Northern
  • Blotting, Western
  • CD5 Antigens / analysis
  • Child
  • Child, Preschool
  • Chromosomes, Human, Pair 13 / genetics*
  • Gene Deletion*
  • Gene Expression Regulation, Leukemic*
  • Humans
  • Hybrid Cells / chemistry
  • Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
  • MicroRNAs / biosynthesis
  • MicroRNAs / genetics*
  • Middle Aged
  • Neoplasms / genetics
  • Neoplasms / pathology
  • Neoplastic Stem Cells / chemistry
  • Palatine Tonsil / cytology
  • RNA, Neoplasm / biosynthesis
  • RNA, Neoplasm / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic
  • Tumor Cells, Cultured / chemistry

Substances

  • CD5 Antigens
  • MicroRNAs
  • RNA, Neoplasm