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83 results

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Page 1
Construction and use of an adaptive optics two-photon microscope with direct wavefront sensing.
Yao P, Liu R, Broggini T, Thunemann M, Kleinfeld D. Yao P, et al. Nat Protoc. 2023 Dec;18(12):3732-3766. doi: 10.1038/s41596-023-00893-w. Epub 2023 Nov 1. Nat Protoc. 2023. PMID: 37914781 Review.
To obviate this limitation, we shape the focal volume, generated by the excitation beam, by modulating the incident wavefront via guidestar-assisted adaptive optics. Here, we describe the construction, calibration and operation of a two-photon microscope that …
To obviate this limitation, we shape the focal volume, generated by the excitation beam, by modulating the incident wavefront via guidestar- …
A practical guide to scanning light-field microscopy with digital adaptive optics.
Lu Z, Cai Y, Nie Y, Yang Y, Wu J, Dai Q. Lu Z, et al. Nat Protoc. 2022 Sep;17(9):1953-1979. doi: 10.1038/s41596-022-00703-9. Epub 2022 Jun 29. Nat Protoc. 2022. PMID: 35768724 Review.
With the development of a wide variety of animal models in recent years, there is a rapidly growing demand for long-term, high-speed intravital fluorescence imaging to observe intercellular and intracellular interactions in their native states. Scanning light-field microscopy …
With the development of a wide variety of animal models in recent years, there is a rapidly growing demand for long-term, high-speed intravi …
Guide to the construction and use of an adaptive optics two-photon microscope with direct wavefront sensing.
Yao P, Liu R, Broginni T, Thunemann M, Kleinfeld D. Yao P, et al. bioRxiv [Preprint]. 2023 Jan 24:2023.01.24.525307. doi: 10.1101/2023.01.24.525307. bioRxiv. 2023. PMID: 36747816 Free PMC article. Updated. Preprint.
Two-photon microscopy, combined with appropriate optical labeling, has enabled the study of structure and function throughout nervous systems. ...We describe the constructions, calibration, and operation of a two-photon microscopy that incorporates adaptiv …
Two-photon microscopy, combined with appropriate optical labeling, has enabled the study of structure and function throughout …
The Fly Brain Atlas.
Scheffer LK, Meinertzhagen IA. Scheffer LK, et al. Annu Rev Cell Dev Biol. 2019 Oct 6;35:637-653. doi: 10.1146/annurev-cellbio-100818-125444. Epub 2019 Jul 8. Annu Rev Cell Dev Biol. 2019. PMID: 31283380 Review.
The brain's synaptic networks endow an animal with powerfully adaptive biological behavior. ...The last decade has witnessed enormous progress in detailed knowledge of the actual synaptic circuits formed by real neurons. Advances in various brain regio …
The brain's synaptic networks endow an animal with powerfully adaptive biological behavior. ...The last decade has witn …
Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin.
Kwon Y, Hong JH, Kang S, Lee H, Jo Y, Kim KH, Yoon S, Choi W. Kwon Y, et al. Nat Commun. 2023 Jan 6;14(1):105. doi: 10.1038/s41467-022-35738-9. Nat Commun. 2023. PMID: 36609405 Free PMC article.
Myelination processes are closely related to higher brain functions such as learning and memory. While their longitudinal observation has been crucial to understanding myelin-related physiology and various brain disorders, skull opening or thinning has been required …
Myelination processes are closely related to higher brain functions such as learning and memory. While their longitudinal observation …
Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains.
Hung ST, Llobet Rosell A, Jurriens D, Siemons M, Soloviev O, Kapitein LC, Grußmayer K, Neukomm LJ, Verhaegen M, Smith C. Hung ST, et al. Front Neurosci. 2022 Oct 6;16:954949. doi: 10.3389/fnins.2022.954949. eCollection 2022. Front Neurosci. 2022. PMID: 36278016 Free PMC article.
Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. ...Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence dec …
Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise loca …
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping.
Qin Z, She Z, Chen C, Wu W, Lau JKY, Ip NY, Qu JY. Qin Z, et al. Nat Biotechnol. 2022 Nov;40(11):1663-1671. doi: 10.1038/s41587-022-01343-w. Epub 2022 Jun 13. Nat Biotechnol. 2022. PMID: 35697805
Here we present an adaptive optics three-photon microscope based on analog lock-in phase detection for focus sensing and shaping (ALPHA-FSS). ALPHA-FSS accurately measures and effectively compensates for both aberrations and scattering induced by specimens an …
Here we present an adaptive optics three-photon microscope based on analog lock-in phase detection for focus sensing an …
Adaptive optics enables aberration-free single-objective remote focusing for two-photon fluorescence microscopy.
Yang Y, Chen W, Fan JL, Ji N. Yang Y, et al. Biomed Opt Express. 2020 Dec 15;12(1):354-366. doi: 10.1364/BOE.413049. eCollection 2021 Jan 1. Biomed Opt Express. 2020. PMID: 33520387 Free PMC article.
Two-photon fluorescence microscopy has been widely applied to three-dimensional (3D) imaging of complex samples. ...In this paper, using a liquid-crystal spatial light modulator to control the divergence of the excitation beam through a single objective, we systematically …
Two-photon fluorescence microscopy has been widely applied to three-dimensional (3D) imaging of complex samples. ...In this paper, us …
Imaging mitochondria through bone in live mice using two-photon fluorescence microscopy with adaptive optics.
Zheng T, Liversage AR, Tehrani KF, Call JA, Kner PA, Mortensen LJ. Zheng T, et al. Front Neuroimaging. 2023 Feb 16;2:959601. doi: 10.3389/fnimg.2023.959601. eCollection 2023. Front Neuroimaging. 2023. PMID: 37554651 Free PMC article.
INTRODUCTION: Mitochondria are extremely important organelles in the regulation of bone marrow and brain activity. However, live imaging of these subcellular features with high resolution in scattering tissues like brain or bone has proven challenging. METHODS: In t …
INTRODUCTION: Mitochondria are extremely important organelles in the regulation of bone marrow and brain activity. However, live imag …
Adaptive optics light-sheet microscopy based on direct wavefront sensing without any guide star.
Hubert A, Harms F, Juvénal R, Treimany P, Levecq X, Loriette V, Farkouh G, Rouyer F, Fragola A. Hubert A, et al. Opt Lett. 2019 May 15;44(10):2514-2517. doi: 10.1364/OL.44.002514. Opt Lett. 2019. PMID: 31090720
We propose an adaptive optics light-sheet fluorescence microscope (AO-LSFM) for closed-loop aberrations' correction at the emission path, providing intrinsic instrumental simplicity and high accuracy when compared to previously reported schemes. ...The design …
We propose an adaptive optics light-sheet fluorescence microscope (AO-LSFM) for closed-loop aberrations' correction at …
83 results