Format

Send to:

Choose Destination
See comment in PubMed Commons below
Methods Enzymol. 2014;537:31-46. doi: 10.1016/B978-0-12-411619-1.00003-3.

Isolation and study of adipocyte precursors.

Author information

  • 1Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut, USA; Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut, USA.
  • 2Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut, USA; Department of Molecular, Cell, and Developmental Biology, Yale University School of Medicine, New Haven, Connecticut, USA; Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut, USA.
  • 3Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut, USA; Department of Molecular, Cell, and Developmental Biology, Yale University School of Medicine, New Haven, Connecticut, USA; Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut, USA. Electronic address: matthew.rodeheffer@yale.edu.

Abstract

White adipose tissue (WAT) is a heterogeneous tissue composed of lipid-filled adipocytes and several nonadipocyte cell populations, including endothelial, blood, uncharacterized stromal, and adipocyte precursor cells. Although lipid-filled adipocytes account for the majority of WAT volume and mass, nonadipocyte cell populations have critical roles in WAT maintenance, growth, and function. As mature adipocytes are terminally differentiated postmitotic cells, differentiation of adipocyte precursors is required for hyperplastic WAT growth during development and in obesity. In this chapter, we present methods to separate adipocyte precursor cells from other nonadipocyte cell populations within WAT for analysis by flow cytometry or purification by fluorescence-activated cell sorting. Additionally, we provide methods to study the adipogenic capacity of purified adipocyte precursor cells ex vivo.

© 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Adipocyte precursor; Adipogenesis; Adipose tissue; Preadipocytes; Progenitor; Stem cell; Stromal vascular

PMID:
24480340
[PubMed - indexed for MEDLINE]
PMCID:
PMC4276307
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk