Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure

Proc Natl Acad Sci U S A. 1987 May;84(10):3365-9. doi: 10.1073/pnas.84.10.3365.

Abstract

A cDNA encoding the CD2 antigen has been isolated by a highly efficient technique based on transient expression in COS cells and adherence of cells expressing surface antigen to antibody-coated dishes. COS cells expressing a CD2 cDNA isolated by this method readily formed rosettes with sheep as well as human and other mammalian erythrocytes. Pretreatment of transfected COS cells with anti-CD2 antibody, or pretreatment of human erythrocytes with anti-LFA-3 antibody, abolished rosette formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface / genetics*
  • Base Sequence
  • Cell Line
  • Cloning, Molecular*
  • DNA / metabolism
  • Epitopes / analysis
  • Erythrocytes / immunology*
  • Genetic Vectors
  • Humans
  • T-Lymphocytes / immunology*

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface
  • Epitopes
  • DNA

Associated data

  • GENBANK/M16445