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Nat Methods. 2014 Jan;11(1):63-5. doi: 10.1038/nmeth.2727. Epub 2013 Nov 10.

Quantifying the local resolution of cryo-EM density maps.

Author information

  • 1Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA.
  • 21] Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA. [2] Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut, USA.
  • 31] Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA. [2] Department of Diagnostic Radiology, Yale University, New Haven, Connecticut, USA.

Abstract

We propose a definition of local resolution for three-dimensional electron cryo-microscopy (cryo-EM) density maps that uses local sinusoidal features. Our algorithm has no free parameters and is applicable to other imaging modalities, including tomography. By evaluating the local resolution of single-particle reconstructions and subtomogram averages for four example data sets, we report variable resolution across a 4- to 40-Å range.

PMID:
24213166
[PubMed - indexed for MEDLINE]
PMCID:
PMC3903095
Free PMC Article
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