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J Vis Exp. 2013 Feb 11;(72). pii: 50197. doi: 10.3791/50197.

Neonatal subventricular zone electroporation.

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  • 1Department of Neurosurgery and Cellular & Molecular Physiology, Yale University School of Medicine, New Haven, CT, USA.

Abstract

Neural stem cells (NSCs) line the postnatal lateral ventricles and give rise to multiple cell types which include neurons, astrocytes, and ependymal cells. Understanding the molecular pathways responsible for NSC self-renewal, commitment, and differentiation is critical for harnessing their unique potential to repair the brain and better understand central nervous system disorders. Previous methods for the manipulation of mammalian systems required the time consuming and expensive endeavor of genetic engineering at the whole animal level. Thus, the vast majority of studies have explored the functions of NSC molecules in vitro or in invertebrates. Here, we demonstrate the simple and rapid technique to manipulate neonatal NPCs that is referred to as neonatal subventricular zone (SVZ) electroporation. Similar techniques were developed a decade ago to study embryonic NSCs and have aided studies on cortical development. More recently this was applied to study the postnatal rodent forebrain. This technique results in robust labeling of SVZ NSCs and their progeny. Thus, postnatal SVZ electroporation provides a cost and time effective alternative for mammalian NSC genetic engineering.

PMID:
23426329
[PubMed - indexed for MEDLINE]
PMCID:
PMC3601042
Free PMC Article
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