Format

Send to:

Choose Destination
See comment in PubMed Commons below
Behav Genet. 2013 Mar;43(2):168-76. doi: 10.1007/s10519-012-9579-1. Epub 2012 Dec 27.

Comparison of whole-genome DNA methylation patterns in whole blood, saliva, and lymphoblastoid cell lines.

Author information

  • 1Yale University, New Haven, CT 06519, USA.

Abstract

Epigenetic mechanisms, including DNA methylation, that underlie neuropsychiatric conditions have become a promising area of research. Most commonly used DNA sources in such studies are peripheral (whole) blood (WB), saliva (SL), and lymphoblastoid cell lines (LCLs); thus, the question of the consistency of DNA methylation patterns in those cells is of particular interest. To investigate this question we performed comparative analyses of methylation patterns in WB, SL, and LCLs derived from the same individuals, using Illumina HumanMethylation27 BeadChip arrays. Our results showed that DNA methylation patterns in SL are relatively consistent with those in WB, whereas the patterns in LCLs are similarly distinct from both WB and SL. The results indicated that due to multiple random and directed changes in DNA methylation throughout cell culturing, LCLs are not a reliable source of DNA for epigenetic studies and should be used with caution when investigating epigenetic mechanisms underlying biological processes.

PMID:
23269419
[PubMed - indexed for MEDLINE]
PMCID:
PMC3577999
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer Icon for PubMed Central
    Loading ...
    Write to the Help Desk